Clinical P. Aeruginosa Prophages: Insights Into Their Role Via Their Activity, Abundance, Persistence

Ifigeneia Kyrkou*, Jennifer Bartell, Cédric Lood, Rasmus Marvig, Rob Lavigne, Helle Krogh Johansen, Søren Molin

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

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Abstract

It remains unclear how much the accessory genome contributes in the complex processes of establishment and virulence of bacterial infections. P. aeruginosa is one of the most common opportunistic human pathogens and can establish difficult-to-eradicate infections. Genome-integrated viruses, known as prophages, are frequent components of this bacterium’s large accessory genome and can contribute to the virulence of P. aeruginosa. However, systematic interpretations of the contributing role of prophages in the evolution and fitness of the ubiquitous P. aeruginosa in its diverse niches are still in their infancy. This study provides insights into these roles by exploring the activity, abundance and persistence of prophages belonging to P. aeruginosa from the cystic fibrosis (CF) lung. We selected a cohort of 12 CF patients with a high-resolution history of difficult-to-eradicate P. aeruginosa infections. Nanopore technology was used to sequence high-contiguity
genomes of one early isolate per patient. Subsequently, we applied a strategy that combined bioinformatics, antibiotic-assisted inductions, lysate sequencing and genomics to identify intact prophages in the host genomes and assess their long-term survival in follow-up isolates. From these data, we observed that CF P. aeruginosa genomes harbour a high abundance of intact prophages which can sometimes self-induce. We identified 29 intact prophages with a wide genomic diversity and
some unique prophage genomes with minimum similarity to available genomes. All induced prophages were retraced in follow-up isolates for a tested period of 4 to 9 years with minimal genomic changes. In addition to elucidating the role of prophages in P. aeruginosa, we expect our findings to assist in developing novel diagnostics and phage-based therapies for P. aeruginosa infections.
Original languageEnglish
Title of host publicationThe Danish Microbiological Society Annual Congress 2023 : Abstract book
Number of pages1
PublisherDanish Microbiological Society
Publication date2023
Pages6-6
Article number1
Publication statusPublished - 2023
EventThe Danish Microbiological Society Annual Congress 2023 - Copenhagen, Denmark
Duration: 13 Nov 202313 Nov 2023

Conference

ConferenceThe Danish Microbiological Society Annual Congress 2023
Country/TerritoryDenmark
CityCopenhagen
Period13/11/202313/11/2023

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