Click-Chemistry of Polymersomes on Nanoporous Polymeric Surfaces

Christian Rein; Steen  Nissen; Mariusz  Grzelakowski; Morten Meldal

doi:10.1002/pola.28069

Click-Chemistry of Polymersomes on Nanoporous Polymeric Surfaces

Christian Rein, Steen Nissen, Mariusz Grzelakowski, Morten Meldal

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A facile click chemistry method of immobilizing surface-functionalized polymer vesicles on casted polymeric PAN substrates is described. Microporous PAN membranes were subjected to hydrochloric acid hydrolysis to obtain surface carboxylates. The carboxylic groups were activated with EDC/NHS-solution and were then reacted with propargylamine to introduce alkyne groups for CuAAC reactions. The alkyne functionality of the modified membrane surface was verified by reaction with an azide functional click dye both before and after the immobilization of azide-functionalized ABA vesicles. The efficient postfunctionalization of the membrane with alkyne allowed quantitative coverage of the membrane surface with a polymersome monolayer, as confirmed by immobilization of polymerzomes loaded with a fluorescent dye. Polymersome monolayers immobilized on alkyne functionalized PANmembranes were characterized by cryo-SEM and monolayers were confirmed by atom force microscopy. These methods
opens up new avenues for preparing membrane based filtration and sensor technologies. © 2016 Wiley Periodicals, Inc.

Original language	English
Journal	Journal of Polymer Science. Part A, Polymer Chemistry
Volume	64
Pages (from-to)	2032–2039
ISSN	0887-624X
DOIs	https://doi.org/10.1002/pola.28069
Publication status	Published - 2016
Externally published	Yes

Keywords

Click Chemistry
Membrane technology
Polymersomes
Polymersome self-assembly
Surface modification

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title = "Click-Chemistry of Polymersomes on Nanoporous Polymeric Surfaces",

abstract = "A facile click chemistry method of immobilizing surface-functionalized polymer vesicles on casted polymeric PAN substrates is described. Microporous PAN membranes were subjected to hydrochloric acid hydrolysis to obtain surface carboxylates. The carboxylic groups were activated with EDC/NHS-solution and were then reacted with propargylamine to introduce alkyne groups for CuAAC reactions. The alkyne functionality of the modified membrane surface was verified by reaction with an azide functional click dye both before and after the immobilization of azide-functionalized ABA vesicles. The efficient postfunctionalization of the membrane with alkyne allowed quantitative coverage of the membrane surface with a polymersome monolayer, as confirmed by immobilization of polymerzomes loaded with a fluorescent dye. Polymersome monolayers immobilized on alkyne functionalized PANmembranes were characterized by cryo-SEM and monolayers were confirmed by atom force microscopy. These methodsopens up new avenues for preparing membrane based filtration and sensor technologies. {\textcopyright} 2016 Wiley Periodicals, Inc.",

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author = "Christian Rein and Steen Nissen and Mariusz Grzelakowski and Morten Meldal",

year = "2016",

doi = "10.1002/pola.28069",

language = "English",

volume = "64",

pages = "2032–2039",

journal = "Journal of Polymer Science. Part A, Polymer Chemistry",

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TY - JOUR

T1 - Click-Chemistry of Polymersomes on Nanoporous Polymeric Surfaces

AU - Rein, Christian

AU - Nissen, Steen

AU - Grzelakowski, Mariusz

AU - Meldal, Morten

PY - 2016

Y1 - 2016

N2 - A facile click chemistry method of immobilizing surface-functionalized polymer vesicles on casted polymeric PAN substrates is described. Microporous PAN membranes were subjected to hydrochloric acid hydrolysis to obtain surface carboxylates. The carboxylic groups were activated with EDC/NHS-solution and were then reacted with propargylamine to introduce alkyne groups for CuAAC reactions. The alkyne functionality of the modified membrane surface was verified by reaction with an azide functional click dye both before and after the immobilization of azide-functionalized ABA vesicles. The efficient postfunctionalization of the membrane with alkyne allowed quantitative coverage of the membrane surface with a polymersome monolayer, as confirmed by immobilization of polymerzomes loaded with a fluorescent dye. Polymersome monolayers immobilized on alkyne functionalized PANmembranes were characterized by cryo-SEM and monolayers were confirmed by atom force microscopy. These methodsopens up new avenues for preparing membrane based filtration and sensor technologies. © 2016 Wiley Periodicals, Inc.

AB - A facile click chemistry method of immobilizing surface-functionalized polymer vesicles on casted polymeric PAN substrates is described. Microporous PAN membranes were subjected to hydrochloric acid hydrolysis to obtain surface carboxylates. The carboxylic groups were activated with EDC/NHS-solution and were then reacted with propargylamine to introduce alkyne groups for CuAAC reactions. The alkyne functionality of the modified membrane surface was verified by reaction with an azide functional click dye both before and after the immobilization of azide-functionalized ABA vesicles. The efficient postfunctionalization of the membrane with alkyne allowed quantitative coverage of the membrane surface with a polymersome monolayer, as confirmed by immobilization of polymerzomes loaded with a fluorescent dye. Polymersome monolayers immobilized on alkyne functionalized PANmembranes were characterized by cryo-SEM and monolayers were confirmed by atom force microscopy. These methodsopens up new avenues for preparing membrane based filtration and sensor technologies. © 2016 Wiley Periodicals, Inc.

KW - Click Chemistry

KW - Membrane technology

KW - Polymersomes

KW - Polymersome self-assembly

KW - Surface modification

U2 - 10.1002/pola.28069

DO - 10.1002/pola.28069

M3 - Journal article

SN - 0887-624X

VL - 64

SP - 2032

EP - 2039

JO - Journal of Polymer Science. Part A, Polymer Chemistry

JF - Journal of Polymer Science. Part A, Polymer Chemistry

ER -

Click-Chemistry of Polymersomes on Nanoporous Polymeric Surfaces

Abstract

Keywords

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