Click chemistry based biomolecular conjugation monitoring using surface-enhanced Raman spectroscopy mapping

Mirko Palla, Shiv Kumar, Zengmin Li, Steffen Jockusch, James J. Russo, Jingyue Ju, Filippo Bosco, Tomas Rindzevicius, Tommy Sonne Alstrøm, Michael Stenbæk Schmidt, Anja Boisen

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review


We describe here a novel surface-enhanced Raman spectroscopy (SERS) based technique for monitoring the conjugation of small molecules by the well-known click reaction between an alkyne and azido moiety on the partner molecules. The monitoring principle is based on the loss of the characteristic alkyne/azide Raman signal with triazole formation in the reaction as a function of time. Since these universal Raman reporter groups are specific for click reactions, this method may facilitate a broad range of applications for monitoring the conjugation efficiency of molecules in diverse areas such as bioconjugation, material science or drug discovery. Additionally, as an attractive advantage of this technique, no significant background signal is expected during the measurements, since these signals reside in a Raman silent region of 2000–2300 cm−1, where virtually all biological molecules are transparent.
Original languageEnglish
Title of host publicationProceedings of IEEE Sensors 2016
Number of pages3
Publication date2016
ISBN (Electronic)978-1-4799-8287-5
Publication statusPublished - 2016
EventIEEE Sensors 2016 - Orlando, FL, United States
Duration: 30 Oct 20163 Nov 2016


ConferenceIEEE Sensors 2016
CountryUnited States
CityOrlando, FL


  • Click Chemistry
  • Bioconjugation monitoring
  • surface-enhanced Raman spectroscopy
  • Statistical quantification
  • Biosensing


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