Abstract
Picornaviral proteinases are responsible for maturation cleavages
of the viral polyprotein, but also catalyze the degradation of
cellular targets. Using graphical visualization techniques and
neural network algorithms, we have investigated the sequence
specificity of the two proteinases
2Apro and
3Cpro. The cleavage of VP0
(giving rise to VP2 and VP4), which is carried out by a so-far
unknown proteinase, was also examined. In combination with a novel
surface exposure prediction algorithm, our neural network approach
successfully distinguishes known cleavage sites from nocleavage
sites and yields a more consistent definition of features common
to these sites. The method is able to predict experimentally
determined cleavage sites in cellular proteins. We present a list
of mammalian and other proteins that are predicted to be possible
targets for the viral proteinases. Whether these proteins are
indeed cleaved awaits experimental verification. Additionally, we
report several errors detected in the protein databases. A
computer server for prediction of cleavage sites by picornaviral
proteinases is publicly available at the e-mail address
NetPicoRNA@cbs.dtu.dk or via WWW at
http://www.cbs.dtu.dk/services/NetPicoRNA
Original language | English |
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Journal | Protein Science |
Volume | 5 |
Issue number | 11 |
Pages (from-to) | 2203-2216 |
ISSN | 0961-8368 |
Publication status | Published - 1996 |