TY - JOUR
T1 - Cleanup and analysis of sugar phosphates in biological extracts by using solid phase extraction and anion-exchange chromatography with pulsed amperometric detection
AU - Smith, Hans Peter
AU - Cohen, A.
AU - Buttler, T.
AU - Nielsen, Jens Bredal
AU - Olsson, Lisbeth
PY - 1998
Y1 - 1998
N2 - A cleanup method based on anion-exchange solid-phase extraction (SPE) was developed to render biological extracts suitable for the analysis of hexose phosphates with a modified anion-exchange chromatography method and pulsed amperometric detection. The method was applied to cell extracts of Saccharomyces cerevisiae obtained by using cold methanol as quenching agent and chloroform as extraction solvent. It was shown that pretreatment of the cell extract with SPE markedly improved the quality of the liquid chromatography analysis with recoveries of the sugar phosphates close to 100%. Furthermore, the method allowed for sample enrichment and the original extraction procedure could be simplified by implementing SPE early in the extraction protocol. (C) 1998 Academic Press.
AB - A cleanup method based on anion-exchange solid-phase extraction (SPE) was developed to render biological extracts suitable for the analysis of hexose phosphates with a modified anion-exchange chromatography method and pulsed amperometric detection. The method was applied to cell extracts of Saccharomyces cerevisiae obtained by using cold methanol as quenching agent and chloroform as extraction solvent. It was shown that pretreatment of the cell extract with SPE markedly improved the quality of the liquid chromatography analysis with recoveries of the sugar phosphates close to 100%. Furthermore, the method allowed for sample enrichment and the original extraction procedure could be simplified by implementing SPE early in the extraction protocol. (C) 1998 Academic Press.
U2 - 10.1006/abio.1998.2714
DO - 10.1006/abio.1998.2714
M3 - Journal article
SN - 0003-2697
VL - 261
SP - 36
EP - 42
JO - Analytical Biochemistry
JF - Analytical Biochemistry
ER -