Reliable test methods for checking cleanliness are needed to evaluate and validate the cleaning process of fermentation tanks. Pilot scale tanks were used to test the applicability of various methods for this purpose. The methods found to be suitable for validation of the clenlinees were visula observtion of a flourorescent indicator using UV light, a contact agar method and culturing based on swabbing and swiping with non-woven cloths. This can be supported by use of Computation Fluid Dynamics CFD.
- tank cleaning
- microbial test methods