Classical swine fever virus detection: results of a real-time reverse transcription polymerase chain reaction ring trial conducted in the framework of the European network of excellence for epizootic disease diagnosis and control

Bernd Hoffmann, Sandra Blome, Paolo Bonilauri, Jovita Fernández-Piñero, Irene Greiser-Wilke, Andy Haegeman, Mats Isaksson, Frank Koenen, Neil LeBlanc, Immanuel Leifer, Marie-Frederique Le Potier, Willie Loeffen, Thomas Bruun Rasmussen, Tomasz Stadejek, Karl Ståhl, Marylène Tignon, Åse Uttenthal, Wim van der Poel, Martin Beer

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    Abstract

    The current study reports on a real-time reverse transcription polymerase chain reaction (real-time RT-PCR) ring trial for the detection of Classical swine fever virus (CSFV) genomic RNA undertaken by 10 European laboratories. All laboratories were asked to use their routine in-house real-time RT-PCR protocols and a standardized protocol commonly used by the Friedrich-Loeffler-Institute (FLI) on a panel of well-characterized samples. In general, all participants produced results within the acceptable range. The FLI assay, several in-house assays, and the commercial kits had high analytical sensitivity and specificity values. Nevertheless, some in-house systems had unspecific reactions or suboptimal sensitivity with only a single CSFV genotype. Follow-up actions involved either improvement of suboptimal assays or replacement of specific laboratory assays with the FLI protocol, with or without modifications. In conclusion, the ring trial showed reliability of classical swine fever diagnosis on an international level and helped to optimize CSFV-specific RT-PCR diagnostics.
    Original languageEnglish
    JournalJournal of Veterinary Diagnostic Investigation
    Volume23
    Issue number5
    Pages (from-to)999-1004
    ISSN1040-6387
    DOIs
    Publication statusPublished - 2011

    Keywords

    • Polymerase chain reaction
    • Ring trial
    • Classical swine fever virus

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