Circulating Levels of MicroRNA from Children with Newly Diagnosed Type 1 Diabetes and Healthy Controls: Evidence That miR-25 Associates to Residual Beta-Cell Function and Glycaemic Control during Disease Progression: evidence that miR-25 associates to residual beta-cell function and glycaemic control during disease progression

Lotte B. Nielsen, Cheng Wang, Kaspar Sorensen, Claus Heiner Bang-Berthelsen, Lars Hansen, Marie-Louise M. Andersen, Philip Hougaard, Anders Christian Juul, Chen-Yu Zhang, Flemming Pociot, Henrik B. Mortensen

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Abstract

This study aims to identify key miRNAs in circulation, which predict ongoing beta-cell destruction and regeneration in children with newly diagnosed Type 1 Diabetes (T1D). We compared expression level of sera miRNAs from new onset T1D children and age-matched healthy controls and related the miRNAs expression levels to beta-cell function and glycaemic control. Global miRNA sequencing analyses were performed on sera pools from two T1D cohorts (n = 275 and 129, resp.) and one control group (n = 151). We identified twelve upregulated human miRNAs in T1D patients (miR-152, miR-30a-5p, miR-181a, miR-24, miR-148a, miR-210, miR-27a, miR-29a, miR-26a, miR-27b, miR-25, miR-200a); several of these miRNAs were linked to apoptosis and beta-cell networks. Furthermore, we identified miR-25 as negatively associated with residual beta-cell function (est.: -0.12, P = 0.0037), and positively associated with glycaemic control (HbA1c) (est.: 0.11, P = 0.0035) 3 months after onset. In conclusion this study demonstrates that miR-25 might be a "tissue-specific" miRNA for glycaemic control 3 months after diagnosis in new onset T1D children and therefore supports the role of circulating miRNAs as predictive biomarkers for tissue physiopathology and potential intervention targets.
Original languageEnglish
Article number896362
JournalExperimental Diabetes Research
Volume2012
Number of pages7
ISSN2314-6745
DOIs
Publication statusPublished - 2012

Keywords

  • Medicine (all)
  • Endocrinology, Diabetes and Metabolism
  • Music
  • biological marker
  • hemoglobin A1c
  • microRNA
  • microRNA 148a
  • microRNA 152
  • microRNA 181a
  • microRNA 200a
  • microRNA 210
  • microRNA 24
  • microRNA 25
  • microRNA 27a
  • microRNA 27b
  • microRNA 29a
  • microRNA 30a
  • microRNA 5p
  • unclassified drug
  • MIRN25 microRNA, human
  • apoptosis
  • article
  • cell function
  • child
  • controlled study
  • disease course
  • glycemic control
  • human
  • insulin dependent diabetes mellitus
  • major clinical study
  • pancreas islet beta cell
  • pathophysiology
  • priority journal
  • protein expression
  • protein function
  • RNA sequence
  • upregulation
  • adolescent
  • blood
  • case control study
  • cohort analysis
  • cytology
  • Denmark
  • DNA sequence
  • female
  • glucose blood level
  • hyperglycemia
  • male
  • metabolism
  • remission
  • Adolescent
  • Biological Markers
  • Blood Glucose
  • Case-Control Studies
  • Child
  • Cohort Studies
  • Diabetes Mellitus, Type 1
  • Disease Progression
  • Female
  • Humans
  • Hyperglycemia
  • Insulin-Secreting Cells
  • Male
  • MicroRNAs
  • Remission Induction
  • Sequence Analysis, DNA
  • Up-Regulation
  • Diseases of the endocrine glands. Clinical endocrinology
  • RC648-665
  • Specialties of internal medicine
  • RC581-951
  • Internal medicine
  • RC31-1245
  • Medicine
  • R
  • DOAJ:Internal medicine
  • DOAJ:Medicine (General)
  • DOAJ:Health Sciences
  • ENDOCRINOLOGY
  • MEDICINE,
  • CANCER-DETECTION
  • SERUM
  • BIOMARKERS
  • EXPRESSION
  • APOPTOSIS
  • AGE
  • Biomarkers

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