Circulating Extracellular microRNA in Systemic Autoimmunity

Niels H. H. Heegaard, Anting Liu Carlsen, Kerstin Skovgaard, Peter M. H. Heegaard

    Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

    Abstract

    MicroRNAs (miRNAs) are differentially regulated in healthy, activated, inflamed, neoplastic, or otherwise pathological cells and tissues. While their main functions are executed intracellularly, many miRNAs can reproducibly be detected extracellularly in plasma and serum. This circulating, extracellular miRNA is protected against degradation by complexation with carrier proteins and/or by being enclosed in subcellular membrane vesicles. This, together with their tissue- and disease-specific expression, has fuelled the interest in using circulating microRNA profiles as harbingers of disease, i.e., as diagnostic analytes and as clues to dysregulated pathways in disease. Many studies show that inflammation and immune dysregulation, e.g., in autoimmune diseases, are associated with distinct miRNA expression changes in targeted tissues and in innate and adaptive immunity cells such as lymphocytes, natural killer cells, neutrophil granulocytes, and monocyte-macrophages. Exploratory studies (only validated in a few cases) also show that specific profiles of circulating miRNAs are associated with different systemic autoimmune diseases including systemic lupus erythematosus (SLE), systemic sclerosis, and rheumatoid arthritis. Even though the link between cellular alterations and extracellular profiles is still unpredictable, the data suggest that circulating miRNAs in autoimmunity may become diagnostically useful. Here, we review important circulating miRNAs in animal models of inflammation and in systemic autoimmunity and summarize some proposed functions of miRNAs in immune regulation and dysregulation. We conclude that the studies suggest new hypotheses and additional experiments, and that further diagnostic development is highly dependent on analytical method development and on obtaining sufficient numbers of uniformly processed samples from clinically well-characterized patients and controls.
    Original languageEnglish
    Title of host publicationCirculating microRNAs in Disease Diagnostics and their Potential Biological Relevance
    Number of pages25
    Volume106
    PublisherSpringer
    Publication date2015
    Pages171-195
    Chapter8
    ISBN (Print)978-3-0348-0953-5
    ISBN (Electronic)978-3-0348-0955-9
    DOIs
    Publication statusPublished - 2015

    Keywords

    • Animal models
    • Autoimmune disease
    • Circulating microRNA
    • Immune system
    • Immunomodulation

    Cite this

    Heegaard, N. H. H., Carlsen, A. L., Skovgaard, K., & Heegaard, P. M. H. (2015). Circulating Extracellular microRNA in Systemic Autoimmunity. In Circulating microRNAs in Disease Diagnostics and their Potential Biological Relevance (Vol. 106, pp. 171-195). Springer. https://doi.org/10.1007/978-3-0348-0955-9_8