Chromosome landing at the ¤Mla¤ locus in barley (¤Hordeum vulgare¤ L.) by means of high-resolution mapping with AFLP markers

G. Schwarz; W. Michalek; V. Mohler; G. Wenzel; A. Jahoor

doi:10.1007/s001220051100

Chromosome landing at the ¤Mla¤ locus in barley (¤Hordeum vulgare¤ L.) by means of high-resolution mapping with AFLP markers

G. Schwarz, W. Michalek, V. Mohler, G. Wenzel, A. Jahoor

Research output: Contribution to journal › Journal article › Research

Abstract

The complex Mla locus of barley determines resistance to the powdery mildew pathogen Erysiphe graminis f. sp. hol dei. With a view towards gene isolation, a population consisting of 950 F-2 individuals derived from a cross between the near-isogenic lines 'P01' (Mla1) and 'P10' (Mla12) was used to construct a high-resolution map of the Mla region. A fluorescence-based AFLP technique and bulked segregant analysis were applied to screen for polymorphic, tightly linked AFLP markers, Three AFLP markers were selected as suitable for a chromosome-landing strategy. One of these AFLP markers and a closely linked RFLP marker were converted into sequence-specific PCR markers. PCR-based screening of approximately 70 000 yeast artificial chromosome (YAC) clones revealed three identical YACs harbouring the Mla locus. Terminal insert sequences were obtained using inverse PCR. The derived STS marker from the right YAC end-clone was mapped distal to the Mla locus.

Original language	English
Journal	Theoretical and Applied Genetics
Volume	98
Issue number	3-4
Pages (from-to)	521-530
ISSN	0040-5752
DOIs	https://doi.org/10.1007/s001220051100
Publication status	Published - 1999

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title = "Chromosome landing at the ¤Mla¤ locus in barley (¤Hordeum vulgare¤ L.) by means of high-resolution mapping with AFLP markers",

abstract = "The complex Mla locus of barley determines resistance to the powdery mildew pathogen Erysiphe graminis f. sp. hol dei. With a view towards gene isolation, a population consisting of 950 F-2 individuals derived from a cross between the near-isogenic lines 'P01' (Mla1) and 'P10' (Mla12) was used to construct a high-resolution map of the Mla region. A fluorescence-based AFLP technique and bulked segregant analysis were applied to screen for polymorphic, tightly linked AFLP markers, Three AFLP markers were selected as suitable for a chromosome-landing strategy. One of these AFLP markers and a closely linked RFLP marker were converted into sequence-specific PCR markers. PCR-based screening of approximately 70 000 yeast artificial chromosome (YAC) clones revealed three identical YACs harbouring the Mla locus. Terminal insert sequences were obtained using inverse PCR. The derived STS marker from the right YAC end-clone was mapped distal to the Mla locus. ",

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author = "G. Schwarz and W. Michalek and V. Mohler and G. Wenzel and A. Jahoor",

year = "1999",

doi = "10.1007/s001220051100",

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T1 - Chromosome landing at the ¤Mla¤ locus in barley (¤Hordeum vulgare¤ L.) by means of high-resolution mapping with AFLP markers

AU - Schwarz, G.

AU - Michalek, W.

AU - Mohler, V.

AU - Wenzel, G.

AU - Jahoor, A.

PY - 1999

Y1 - 1999

N2 - The complex Mla locus of barley determines resistance to the powdery mildew pathogen Erysiphe graminis f. sp. hol dei. With a view towards gene isolation, a population consisting of 950 F-2 individuals derived from a cross between the near-isogenic lines 'P01' (Mla1) and 'P10' (Mla12) was used to construct a high-resolution map of the Mla region. A fluorescence-based AFLP technique and bulked segregant analysis were applied to screen for polymorphic, tightly linked AFLP markers, Three AFLP markers were selected as suitable for a chromosome-landing strategy. One of these AFLP markers and a closely linked RFLP marker were converted into sequence-specific PCR markers. PCR-based screening of approximately 70 000 yeast artificial chromosome (YAC) clones revealed three identical YACs harbouring the Mla locus. Terminal insert sequences were obtained using inverse PCR. The derived STS marker from the right YAC end-clone was mapped distal to the Mla locus.

AB - The complex Mla locus of barley determines resistance to the powdery mildew pathogen Erysiphe graminis f. sp. hol dei. With a view towards gene isolation, a population consisting of 950 F-2 individuals derived from a cross between the near-isogenic lines 'P01' (Mla1) and 'P10' (Mla12) was used to construct a high-resolution map of the Mla region. A fluorescence-based AFLP technique and bulked segregant analysis were applied to screen for polymorphic, tightly linked AFLP markers, Three AFLP markers were selected as suitable for a chromosome-landing strategy. One of these AFLP markers and a closely linked RFLP marker were converted into sequence-specific PCR markers. PCR-based screening of approximately 70 000 yeast artificial chromosome (YAC) clones revealed three identical YACs harbouring the Mla locus. Terminal insert sequences were obtained using inverse PCR. The derived STS marker from the right YAC end-clone was mapped distal to the Mla locus.

KW - Planteproduktion og stofomsætning

U2 - 10.1007/s001220051100

DO - 10.1007/s001220051100

M3 - Journal article

SN - 0040-5752

VL - 98

SP - 521

EP - 530

JO - Theoretical and Applied Genetics

JF - Theoretical and Applied Genetics

IS - 3-4

ER -

Chromosome landing at the ¤Mla¤ locus in barley (¤Hordeum vulgare¤ L.) by means of high-resolution mapping with AFLP markers

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