TY - JOUR
T1 - Chitosan-coated Oleaginous Microalgae-Fungal Pellets for Improved Bioremediation of Non-sterile Secondary Effluent and Application in Carbon Dioxide Sequestration in Bubble Column Photobioreactors
AU - Maneechote, Wageeporn
AU - Cheirsilp, Benjamas
AU - Angelidaki, Irini
AU - Suyotha, Wasana
AU - Boonsawang, Piyarat
PY - 2023
Y1 - 2023
N2 - Oleaginous microalga Scenedesmus sp. SPP was rapidly immobilized in oleaginous fungal pellets by their opposite-surface-charges. Microalgae-fungal (MF) pellets were more effective in bioremediation of non-sterile secondary effluent than mono-culture. The optimal hydraulic retention time for dual bioremediation in semi-continuous mode was 72 h. The MF pellets coated with 0.4%-chitosan improved removal efficiencies of COD, total nitrogen (TN), and total phosphorus (TP) up to 96.2±0.0%, 88.2±2.8% and 71.5±0.7%, respectively, likely because of better cell retention and more nutrient adsorption and assimilation. Dual bioremediation by coated MF pellets was also successfully scaled up in 30-L bubble-column photobioreactors with improved COD, TN, and TP removal efficiencies of 98.5±0.0%, 90.2±0.0% and 79.5±2.1%, respectively. This system also effectively removed CO2 from simulated flue gas at 71.2±0.4% and produced biomass with high lipid content. These results highlight the effectiveness of bio-immobilization by fungal pellets; chitosan coating; and their practical applications in bioremediation and CO2 sequestration.
AB - Oleaginous microalga Scenedesmus sp. SPP was rapidly immobilized in oleaginous fungal pellets by their opposite-surface-charges. Microalgae-fungal (MF) pellets were more effective in bioremediation of non-sterile secondary effluent than mono-culture. The optimal hydraulic retention time for dual bioremediation in semi-continuous mode was 72 h. The MF pellets coated with 0.4%-chitosan improved removal efficiencies of COD, total nitrogen (TN), and total phosphorus (TP) up to 96.2±0.0%, 88.2±2.8% and 71.5±0.7%, respectively, likely because of better cell retention and more nutrient adsorption and assimilation. Dual bioremediation by coated MF pellets was also successfully scaled up in 30-L bubble-column photobioreactors with improved COD, TN, and TP removal efficiencies of 98.5±0.0%, 90.2±0.0% and 79.5±2.1%, respectively. This system also effectively removed CO2 from simulated flue gas at 71.2±0.4% and produced biomass with high lipid content. These results highlight the effectiveness of bio-immobilization by fungal pellets; chitosan coating; and their practical applications in bioremediation and CO2 sequestration.
KW - Bioremediation
KW - Fungal pellets
KW - Immobilization
KW - Microalgae
KW - Secondary effluent
U2 - 10.1016/j.biortech.2023.128675
DO - 10.1016/j.biortech.2023.128675
M3 - Journal article
C2 - 36706817
SN - 0960-8524
VL - 372
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 128675
ER -