Chemoenzymatic synthesis of branched oligo- and polysaccharides as potential substrates for starch active enzymes

Lionel Greffe, Morten T. Jensen, Claude Bosso, Birte Svensson, Hugues Driguez

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Oligo- and polysaccharides embodying the alpha-maltotriosyl-6(II)-maltotetraosyl structure were readily synthesized by transglycosylation of maltosyl fluoride onto panose and pullulan catalysed by the bacterial transglycosylase cyclodextrin glycosyltransferase (CGTase). The two products obtained proved useful for increasing the knowledge of substrate binding and processing at the active site of barley limit dextrinase that is involved in the metabolism of amylopectin by acting upon its branch points.
Original languageEnglish
JournalChemBioChem
Volume4
Issue number12
Pages (from-to)100-104
ISSN1439-4227
DOIs
Publication statusPublished - 2003
Externally publishedYes

Cite this

Greffe, Lionel ; Jensen, Morten T. ; Bosso, Claude ; Svensson, Birte ; Driguez, Hugues. / Chemoenzymatic synthesis of branched oligo- and polysaccharides as potential substrates for starch active enzymes. In: ChemBioChem. 2003 ; Vol. 4, No. 12. pp. 100-104.
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abstract = "Oligo- and polysaccharides embodying the alpha-maltotriosyl-6(II)-maltotetraosyl structure were readily synthesized by transglycosylation of maltosyl fluoride onto panose and pullulan catalysed by the bacterial transglycosylase cyclodextrin glycosyltransferase (CGTase). The two products obtained proved useful for increasing the knowledge of substrate binding and processing at the active site of barley limit dextrinase that is involved in the metabolism of amylopectin by acting upon its branch points.",
author = "Lionel Greffe and Jensen, {Morten T.} and Claude Bosso and Birte Svensson and Hugues Driguez",
year = "2003",
doi = "10.1002/cbic.200300692",
language = "English",
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pages = "100--104",
journal = "ChemBioChem",
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Chemoenzymatic synthesis of branched oligo- and polysaccharides as potential substrates for starch active enzymes. / Greffe, Lionel; Jensen, Morten T.; Bosso, Claude; Svensson, Birte; Driguez, Hugues.

In: ChemBioChem, Vol. 4, No. 12, 2003, p. 100-104.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Chemoenzymatic synthesis of branched oligo- and polysaccharides as potential substrates for starch active enzymes

AU - Greffe, Lionel

AU - Jensen, Morten T.

AU - Bosso, Claude

AU - Svensson, Birte

AU - Driguez, Hugues

PY - 2003

Y1 - 2003

N2 - Oligo- and polysaccharides embodying the alpha-maltotriosyl-6(II)-maltotetraosyl structure were readily synthesized by transglycosylation of maltosyl fluoride onto panose and pullulan catalysed by the bacterial transglycosylase cyclodextrin glycosyltransferase (CGTase). The two products obtained proved useful for increasing the knowledge of substrate binding and processing at the active site of barley limit dextrinase that is involved in the metabolism of amylopectin by acting upon its branch points.

AB - Oligo- and polysaccharides embodying the alpha-maltotriosyl-6(II)-maltotetraosyl structure were readily synthesized by transglycosylation of maltosyl fluoride onto panose and pullulan catalysed by the bacterial transglycosylase cyclodextrin glycosyltransferase (CGTase). The two products obtained proved useful for increasing the knowledge of substrate binding and processing at the active site of barley limit dextrinase that is involved in the metabolism of amylopectin by acting upon its branch points.

U2 - 10.1002/cbic.200300692

DO - 10.1002/cbic.200300692

M3 - Journal article

VL - 4

SP - 100

EP - 104

JO - ChemBioChem

JF - ChemBioChem

SN - 1439-4227

IS - 12

ER -