Chemoenzymatic synthesis of branched oligo- and polysaccharides as potential substrates for starch active enzymes

Lionel Greffe, Morten T. Jensen, Claude Bosso, Birte Svensson, Hugues Driguez

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Oligo- and polysaccharides embodying the alpha-maltotriosyl-6(II)-maltotetraosyl structure were readily synthesized by transglycosylation of maltosyl fluoride onto panose and pullulan catalysed by the bacterial transglycosylase cyclodextrin glycosyltransferase (CGTase). The two products obtained proved useful for increasing the knowledge of substrate binding and processing at the active site of barley limit dextrinase that is involved in the metabolism of amylopectin by acting upon its branch points.
Original languageEnglish
JournalChemBioChem
Volume4
Issue number12
Pages (from-to)100-104
ISSN1439-4227
DOIs
Publication statusPublished - 2003
Externally publishedYes

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