A method is described for the evaluation of the peroxide value (PV, meq. O-2 kg(-1)) in olive oil The method is based on the chemiluminogenic energy-transfer reaction of bis(2,4,6-(trichlorophenyl)oxalate (TCPO) with hydrogen peroxide or total peroxides in the presence of Mn(II) as catalyst and 9, 10-dimethylanthracene as fluorophore. The procedure developed allows the evaluation of PV within the range of 0.6-100 meq. O-2 kg(-1) (CL intensity = 1.76 x PV (meq. O-2 kg(-1)) + 23.2, r(2) = 0.994, n = 9) and relative standard deviation within the range 1-5% by using a simple manual measurement. (C) 2004 Elsevier B.V. All rights reserved.