In order to trigger expression of “silent” genes we are currently pursuing several approaches; i) stimulation of A. nidulans wild type strains by culturing on different complex media to provoke induction of the secondary metabolism; ii) over expression of transcription factors encoding genes that are present in PKS gene clusters; iii) modification of chromatin structure regulation by knock out of histone H3 lysine methylation; iv) more random induction of secondary metabolism through heterologous expression of regulatory genes from other filamentous fungi using A. niger as test case.
To facilitate the linking of new compounds to genes we have made a collection of mutant strains where all thirty-two individual genes predicted to encode polyketide synthases have been individually been deleted. This presentation will highlight our recent linking of secondary metabolites in A. nidulans to genes, and in particular describe some recent work on characterization of ANID_6448 and associated genes responsible for biosynthesis of 3-methyl-orsellinic acid and derived products.
|Publication status||Published - 2012|
|Event||Directing Biosynthesis III - University of Nottingham, Nottingham, United Kingdom|
Duration: 19 Sep 2012 → 21 Sep 2012
|Conference||Directing Biosynthesis III|
|Location||University of Nottingham|
|Period||19/09/2012 → 21/09/2012|