Characterization of water-forming NADH oxidases for co-factor regeneration

Gustav Rehn, Asbjørn Toftgaard Pedersen, Simon J. Charnock, John Woodley

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Traditional chemical methods for alcohol oxidation are often associated with issues such as high consumption of expensive oxidizing agents, generation of metal waste and the use of environmentally undesirable organic solvents. Developing green, selective catalysts is therefore important from an environmentaland economic perspective [1].
Alcohol dehydrogenases (ADH) offer one such alternative. However, the reaction requires the oxidized nicotinamide co-factor (NAD+) that must be recycled due to its high cost contribution. One regeneration method that offers certain advantages is the oxidation of NADH using water forming NADH oxidases (NOX-2). The implementation of the ADH/NOX system for alcohol oxidation, however, requires consideration of several different issues. Enzyme activity and stability at relevant pH and temperature conditions, but also the tolerance to the substrates and products present (alcohols and aldehydes) are important properties to characterize. Importantly, inactivation by gas-liquid interfaces has been reported for some enzymes, such as the NOX from Lactococcuslactis[2]. Thus, investigating the sensitivity to bubbling is also highly important from a process development perspective.
Original languageEnglish
Publication date2016
Number of pages1
Publication statusPublished - 2016
EventBiocat 2016 - Hamburg University of Technology, Hamburg, Germany
Duration: 28 Aug 20161 Sep 2016


ConferenceBiocat 2016
LocationHamburg University of Technology

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