Characterization of thin gelatin hydrogel membranes with balloon properties for dynamic tissue engineering

Morten Leth Jepsen, Line Hagner Nielsen, Anja Boisen, Kristoffer Almdal, Martin Dufva*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

154 Downloads (Pure)


Cell or tissue stretching and strain are present in any in vivo environment, but is difficult to reproduce in vitro. Here, we describe a simple method for casting a thin (about 500 μm) and soft (about 0.3 kPa) hydrogel of gelatin and a method for characterizing the mechanical properties of the hydrogel simply by changing pressure with a water column. The gelatin is crosslinked with mTransglutaminase and the area of the resulting hydrogel can be increased up 13-fold by increasing the radial water pressure. This is far beyond physiological stretches observed in vivo. Actuating the hydrogel with a radial force achieves both information about stiffness, stretchability, and contractability, which are relevant properties for tissue engineering purposes. Cells could be stretched and contracted using the gelatin membrane. Gelatin is a commonly used polymer for hydrogels in tissue engineering, and the discovered reversible stretching is particularly interesting for organ modeling applications.

Original languageEnglish
Article numbere23241
Issue number1
Publication statusPublished - 2019


  • Biomimetics
  • Gelatin
  • Hydrogels
  • Rheology


Dive into the research topics of 'Characterization of thin gelatin hydrogel membranes with balloon properties for dynamic tissue engineering'. Together they form a unique fingerprint.

Cite this