TY - JOUR
T1 - Characterization of the LM5 pectic galactan epitope with synthetic analogues of β-1,4-d-galactotetraose
AU - Andersen, Mathias Christian Franch
AU - Boos, Irene
AU - Marcus, Susan E.
AU - Kračun, Stjepan K.
AU - Rydahl, Maja Gro
AU - Willats, William G.T.
AU - Knox, J. Paul
AU - Clausen, Mads Hartvig
PY - 2016
Y1 - 2016
N2 - Plant cell wall glycans are important polymers that are crucial to plant development and serve as an important source of sustainable biomass. The study of polysaccharides in the plant cell wall relies heavily on monoclonal antibodies for localization and visualization of glycans, using e.g. Immunofluorescent microscopy. Here, we describe the detailed epitope mapping of the mab LM5 that is shown to bind to a minimum of three sugar residues at the non-reducing end of linear beta-1,4-linked galactan. The study uses de novo synthetic analogs of galactans combined with carbohydrate microarray and competitive inhibition ELISA for analysis of antibody-carbohydrate interactions.
AB - Plant cell wall glycans are important polymers that are crucial to plant development and serve as an important source of sustainable biomass. The study of polysaccharides in the plant cell wall relies heavily on monoclonal antibodies for localization and visualization of glycans, using e.g. Immunofluorescent microscopy. Here, we describe the detailed epitope mapping of the mab LM5 that is shown to bind to a minimum of three sugar residues at the non-reducing end of linear beta-1,4-linked galactan. The study uses de novo synthetic analogs of galactans combined with carbohydrate microarray and competitive inhibition ELISA for analysis of antibody-carbohydrate interactions.
U2 - 10.1016/j.carres.2016.10.012
DO - 10.1016/j.carres.2016.10.012
M3 - Journal article
C2 - 27855335
SN - 0008-6215
VL - 436
SP - 36
EP - 40
JO - Carbohydrate Research
JF - Carbohydrate Research
ER -