Characterization of the LM5 pectic galactan epitope with synthetic analogues of β-1,4-d-galactotetraose

Mathias Christian Franch Andersen; Irene Boos; Susan E. Marcus; Stjepan K. Kračun; Maja Gro Rydahl; William G.T. Willats; J. Paul Knox; Mads Hartvig Clausen

doi:10.1016/j.carres.2016.10.012

Characterization of the LM5 pectic galactan epitope with synthetic analogues of β-1,4-d-galactotetraose

Mathias Christian Franch Andersen, Irene Boos, Susan E. Marcus, Stjepan K. Kračun, Maja Gro Rydahl, William G.T. Willats, J. Paul Knox, Mads Hartvig Clausen

Department of Chemistry

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Abstract

Plant cell wall glycans are important polymers that are crucial to plant development and serve as an important source of sustainable biomass. The study of polysaccharides in the plant cell wall relies heavily on monoclonal antibodies for localization and visualization of glycans, using e.g. Immunofluorescent microscopy. Here, we describe the detailed epitope mapping of the mab LM5 that is shown to bind to a minimum of three sugar residues at the non-reducing end of linear beta-1,4-linked galactan. The study uses de novo synthetic analogs of galactans combined with carbohydrate microarray and competitive inhibition ELISA for analysis of antibody-carbohydrate interactions.

Original language	English
Journal	Carbohydrate Research
Volume	436
Pages (from-to)	36–40
ISSN	0008-6215
DOIs	https://doi.org/10.1016/j.carres.2016.10.012
Publication status	Published - 2016

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Characterization of the LM5 pectic galactan epitope with synthetic analogues of 1 4 D galactotetraose postprintAccepted author manuscript, 403 KB

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title = "Characterization of the LM5 pectic galactan epitope with synthetic analogues of β-1,4-d-galactotetraose",

abstract = "Plant cell wall glycans are important polymers that are crucial to plant development and serve as an important source of sustainable biomass. The study of polysaccharides in the plant cell wall relies heavily on monoclonal antibodies for localization and visualization of glycans, using e.g. Immunofluorescent microscopy. Here, we describe the detailed epitope mapping of the mab LM5 that is shown to bind to a minimum of three sugar residues at the non-reducing end of linear beta-1,4-linked galactan. The study uses de novo synthetic analogs of galactans combined with carbohydrate microarray and competitive inhibition ELISA for analysis of antibody-carbohydrate interactions.",

author = "Andersen, {Mathias Christian Franch} and Irene Boos and Marcus, {Susan E.} and Kra{\v c}un, {Stjepan K.} and Rydahl, {Maja Gro} and Willats, {William G.T.} and Knox, {J. Paul} and Clausen, {Mads Hartvig}",

year = "2016",

doi = "10.1016/j.carres.2016.10.012",

language = "English",

volume = "436",

pages = "36–40",

journal = "Carbohydrate Research",

issn = "0008-6215",

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TY - JOUR

T1 - Characterization of the LM5 pectic galactan epitope with synthetic analogues of β-1,4-d-galactotetraose

AU - Andersen, Mathias Christian Franch

AU - Boos, Irene

AU - Marcus, Susan E.

AU - Kračun, Stjepan K.

AU - Rydahl, Maja Gro

AU - Willats, William G.T.

AU - Knox, J. Paul

AU - Clausen, Mads Hartvig

PY - 2016

Y1 - 2016

N2 - Plant cell wall glycans are important polymers that are crucial to plant development and serve as an important source of sustainable biomass. The study of polysaccharides in the plant cell wall relies heavily on monoclonal antibodies for localization and visualization of glycans, using e.g. Immunofluorescent microscopy. Here, we describe the detailed epitope mapping of the mab LM5 that is shown to bind to a minimum of three sugar residues at the non-reducing end of linear beta-1,4-linked galactan. The study uses de novo synthetic analogs of galactans combined with carbohydrate microarray and competitive inhibition ELISA for analysis of antibody-carbohydrate interactions.

AB - Plant cell wall glycans are important polymers that are crucial to plant development and serve as an important source of sustainable biomass. The study of polysaccharides in the plant cell wall relies heavily on monoclonal antibodies for localization and visualization of glycans, using e.g. Immunofluorescent microscopy. Here, we describe the detailed epitope mapping of the mab LM5 that is shown to bind to a minimum of three sugar residues at the non-reducing end of linear beta-1,4-linked galactan. The study uses de novo synthetic analogs of galactans combined with carbohydrate microarray and competitive inhibition ELISA for analysis of antibody-carbohydrate interactions.

U2 - 10.1016/j.carres.2016.10.012

DO - 10.1016/j.carres.2016.10.012

M3 - Journal article

C2 - 27855335

SN - 0008-6215

VL - 436

SP - 36

EP - 40

JO - Carbohydrate Research

JF - Carbohydrate Research

ER -

Characterization of the LM5 pectic galactan epitope with synthetic analogues of β-1,4-d-galactotetraose

Abstract

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