Characterization of the LM5 pectic galactan epitope with synthetic analogues of β-1,4-d-galactotetraose

Mathias Christian Franch Andersen, Irene Boos, Susan E. Marcus, Stjepan K. Kračun, Maja Gro Rydahl, William G.T. Willats, J. Paul Knox, Mads Hartvig Clausen

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Abstract

Plant cell wall glycans are important polymers that are crucial to plant development and serve as an important source of sustainable biomass. The study of polysaccharides in the plant cell wall relies heavily on monoclonal antibodies for localization and visualization of glycans, using e.g. Immunofluorescent microscopy. Here, we describe the detailed epitope mapping of the mab LM5 that is shown to bind to a minimum of three sugar residues at the non-reducing end of linear beta-1,4-linked galactan. The study uses de novo synthetic analogs of galactans combined with carbohydrate microarray and competitive inhibition ELISA for analysis of antibody-carbohydrate interactions.
Original languageEnglish
JournalCarbohydrate Research
Volume436
Pages (from-to)36–40
ISSN0008-6215
DOIs
Publication statusPublished - 2016

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