Characterization of the glucagon-like peptide-1 receptor in male mouse brain using a novel antibody and in situ hybridization

Casper Bo Jensen, Charles Pyke, Morten Grønbech Rasch, Anders Bjorholm Dahl, Lotte Bjerre Knudsen*, Anna Secher

*Corresponding author for this work

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Glucagon-like peptide-1 (GLP-1) is a physiological regulator of appetite and long-acting GLP-1 receptor agonists (GLP-1RA) lower food intake and bodyweight in both human and animal studies. The effects are mediated through brain GLP-1Rs, and several brain nuclei expressing the GLP-1R may be involved. To date, mapping the complete location of GLP-1R protein in the brain has been challenged by lack of good antibodies and the discrepancy between mRNA and protein especially relevant in neuronal axonal processes. Here, we present a novel and specific monoclonal GLP-1R antibody for immunohistochemistry with murine tissue and show detailed distribution of GLP-1R expression as well as mapping of GLP-1R mRNA by non-radioactive in situ hybridization. Semi-automated image analysis was performed to map the GLP-1R distribution to atlas plates from the Allen Institute of Brain Science (AIBS). The GLP-1R was abundantly expressed in numerous regions including the septal nucleus, the hypothalamus and the brain stem. GLP-1R protein expression was also observed on neuronal projections in brain regions devoid of any mRNA which has not been observed in earlier reports. Taken together, these findings provide new knowledge on GLP-1R expression in neuronal cell bodies and neuronal projections.
Original languageEnglish
Article numberen.2017-00812
JournalJournal of Clinical Endocrinology and Metabolism
Issue number2
Pages (from-to)665–675
Publication statusPublished - 2017


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