Characterization of the CI Repressor Protein Encoded by the Temperate Lactococcal Phage TP901-1

Margit Pedersen, Malgorzata Anna Ligowska, Karin Hammer

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The gene regulatory mechanism determining the developmental pathway of the temperate bacteriophage TP901-1 is regulated by two phage-encoded proteins, CI and MOR. Functional domains of the CI repressor were investigated by introducing linkers of 15 bp at various positions in cI and by limited proteolysis of purified CI protein. We show that insertions of five amino acids at positions in the N-terminal half of CI resulted in mutant proteins that could no longer repress transcription from the lytic promoter, P-L. We confirmed that the N-terminal domain of CI contains the DNA binding site, and we showed that this part of the protein is tightly folded, whereas the central part and the C-terminal part of CI seem to contain more flexible structures. Furthermore, insertions at several different positions in the central part of the CI protein reduced the cooperative binding of CI to the operator sites and possibly altered the interaction with MOR.
    Original languageEnglish
    JournalJournal of Bacteriology
    Volume192
    Issue number8
    Pages (from-to)2102-2110
    ISSN0021-9193
    DOIs
    Publication statusPublished - 2010

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