Characterization of the chemoenzymatic synthesis of N-acetyl-D-neuraminic acid (Neu5Ac)

Simone Blayer; John Woodley; Malcolm D. Lilly; Michael J. Dawson

doi:10.1021/bp9600647

Characterization of the chemoenzymatic synthesis of N-acetyl-D-neuraminic acid (Neu5Ac)

Simone Blayer
, John Woodley
, Malcolm D. Lilly
, Michael J. Dawson

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The characterization of the chemoenzymatic synthesis of N-acetyl-D-neuraminic acid using a base-catalyzed epimerization and Neu5Ac aldolase-catalyzed condensation is presented. The characterization has been used to exemplify a structured approach to biotransformation process design. In addition, the option of integration of the epimerization with the biotransformation (at alkaline pH) was considered. Process operating windows have been generated as a result of this approach, defining possible operating regions for subsequent scale-up.

Original language	English
Journal	Biotechnology Progress
Volume	12
Issue number	6
Pages (from-to)	758-763
ISSN	8756-7938
DOIs	https://doi.org/10.1021/bp9600647
Publication status	Published - 1996
Externally published	Yes

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title = "Characterization of the chemoenzymatic synthesis of N-acetyl-D-neuraminic acid (Neu5Ac)",

abstract = "The characterization of the chemoenzymatic synthesis of N-acetyl-D-neuraminic acid using a base-catalyzed epimerization and Neu5Ac aldolase-catalyzed condensation is presented. The characterization has been used to exemplify a structured approach to biotransformation process design. In addition, the option of integration of the epimerization with the biotransformation (at alkaline pH) was considered. Process operating windows have been generated as a result of this approach, defining possible operating regions for subsequent scale-up. ",

author = "Simone Blayer and John Woodley and Lilly, \{Malcolm D.\} and Dawson, \{Michael J.\}",

year = "1996",

doi = "10.1021/bp9600647",

language = "English",

volume = "12",

pages = "758--763",

journal = "Biotechnology Progress",

issn = "8756-7938",

publisher = "Wiley-Blackwell",

number = "6",

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TY - JOUR

T1 - Characterization of the chemoenzymatic synthesis of N-acetyl-D-neuraminic acid (Neu5Ac)

AU - Blayer, Simone

AU - Woodley, John

AU - Lilly, Malcolm D.

AU - Dawson, Michael J.

PY - 1996

Y1 - 1996

N2 - The characterization of the chemoenzymatic synthesis of N-acetyl-D-neuraminic acid using a base-catalyzed epimerization and Neu5Ac aldolase-catalyzed condensation is presented. The characterization has been used to exemplify a structured approach to biotransformation process design. In addition, the option of integration of the epimerization with the biotransformation (at alkaline pH) was considered. Process operating windows have been generated as a result of this approach, defining possible operating regions for subsequent scale-up.

AB - The characterization of the chemoenzymatic synthesis of N-acetyl-D-neuraminic acid using a base-catalyzed epimerization and Neu5Ac aldolase-catalyzed condensation is presented. The characterization has been used to exemplify a structured approach to biotransformation process design. In addition, the option of integration of the epimerization with the biotransformation (at alkaline pH) was considered. Process operating windows have been generated as a result of this approach, defining possible operating regions for subsequent scale-up.

U2 - 10.1021/bp9600647

DO - 10.1021/bp9600647

M3 - Journal article

SN - 8756-7938

VL - 12

SP - 758

EP - 763

JO - Biotechnology Progress

JF - Biotechnology Progress

IS - 6

ER -

Characterization of the chemoenzymatic synthesis of N-acetyl-D-neuraminic acid (Neu5Ac)

Abstract

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