Abstract
The characterization of the chemoenzymatic synthesis of N-acetyl-D-neuraminic acid using a base-catalyzed epimerization and Neu5Ac aldolase-catalyzed condensation is presented. The characterization has been used to exemplify a structured approach to biotransformation process design. In addition, the option of integration of the epimerization with the biotransformation (at alkaline pH) was considered. Process operating windows have been generated as a result of this approach, defining possible operating regions for subsequent scale-up.
Original language | English |
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Journal | Biotechnology Progress |
Volume | 12 |
Issue number | 6 |
Pages (from-to) | 758-763 |
ISSN | 8756-7938 |
DOIs | |
Publication status | Published - 1996 |
Externally published | Yes |