Characterization of the chemoenzymatic synthesis of N-acetyl-D-neuraminic acid (Neu5Ac)

Simone Blayer, John Woodley, Malcolm D. Lilly, Michael J. Dawson

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The characterization of the chemoenzymatic synthesis of N-acetyl-D-neuraminic acid using a base-catalyzed epimerization and Neu5Ac aldolase-catalyzed condensation is presented. The characterization has been used to exemplify a structured approach to biotransformation process design. In addition, the option of integration of the epimerization with the biotransformation (at alkaline pH) was considered. Process operating windows have been generated as a result of this approach, defining possible operating regions for subsequent scale-up.
Original languageEnglish
JournalBiotechnology Progress
Volume12
Issue number6
Pages (from-to)758-763
ISSN8756-7938
DOIs
Publication statusPublished - 1996
Externally publishedYes

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