Characterization of the AN6448 cluster in Aspergillus nidulans

Jakob Blæsbjerg Nielsen; Marie Louise Klejnstrup; Paiman Khorsand-Jamal; Dorte Koefoed Holm; Michael Lynge Nielsen; Anna Monika Kabat; Christian Rank; Charlotte Held Gotfredsen; T. O. Larsen; Uffe Hasbro Mortensen

doi:10.1055/s-0032-1320546

Characterization of the AN6448 cluster in Aspergillus nidulans

Jakob Blæsbjerg Nielsen, Marie Louise Klejnstrup, Paiman Khorsand-Jamal, Dorte Koefoed Holm, Michael Lynge Nielsen, Anna Monika Kabat, Christian Rank, Charlotte Held Gotfredsen, T. O. Larsen, Uffe Hasbro Mortensen

Department of Chemistry

Research output: Contribution to journal › Conference abstract in journal › Research › peer-review

Abstract

With the aim of mapping the polyketome of A. nidulans we have made a library of strains, which individually overexpress PKS genes from an ectopic locus. A screen of this collection on different media demonstrated that AN6448 leads to production of 3-MOA. An inspection of the DNA sequence surrounding this gene uncovered a putative gene cluster including a gene, AN6446, with homology to transcription factors. Based on this observation, we decided to overexpress AN6446. A qRT-PCR analysis of this strain was used to delineate the borders of the gene cluster as well as to stimulate formation of cichorine and a number of new products, from the gene cluster. Subsequent deletion of all genes allowed several steps in the biosynthetic pathway in this cluster to be clarified.

Original language	English
Journal	Planta Medica
Volume	78
Issue number	11
Pages (from-to)	1113-1113
ISSN	0032-0943
DOIs	https://doi.org/10.1055/s-0032-1320546
Publication status	Published - 2012
Event	International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF - New York, NY, United States Duration: 28 Jul 2012 → 1 Aug 2012

Conference

Conference	International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF
Country/Territory	United States
City	New York, NY
Period	28/07/2012 → 01/08/2012

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title = "Characterization of the AN6448 cluster in Aspergillus nidulans",

abstract = "With the aim of mapping the polyketome of A. nidulans we have made a library of strains, which individually overexpress PKS genes from an ectopic locus. A screen of this collection on different media demonstrated that AN6448 leads to production of 3-MOA. An inspection of the DNA sequence surrounding this gene uncovered a putative gene cluster including a gene, AN6446, with homology to transcription factors. Based on this observation, we decided to overexpress AN6446. A qRT-PCR analysis of this strain was used to delineate the borders of the gene cluster as well as to stimulate formation of cichorine and a number of new products, from the gene cluster. Subsequent deletion of all genes allowed several steps in the biosynthetic pathway in this cluster to be clarified.",

author = "Nielsen, \{Jakob Bl{\ae}sbjerg\} and Klejnstrup, \{Marie Louise\} and Paiman Khorsand-Jamal and Holm, \{Dorte Koefoed\} and Nielsen, \{Michael Lynge\} and Kabat, \{Anna Monika\} and Christian Rank and Gotfredsen, \{Charlotte Held\} and Larsen, \{T. O.\} and Mortensen, \{Uffe Hasbro\}",

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doi = "10.1055/s-0032-1320546",

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volume = "78",

pages = "1113--1113",

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T1 - Characterization of the AN6448 cluster in Aspergillus nidulans

AU - Nielsen, Jakob Blæsbjerg

AU - Klejnstrup, Marie Louise

AU - Khorsand-Jamal, Paiman

AU - Holm, Dorte Koefoed

AU - Nielsen, Michael Lynge

AU - Kabat, Anna Monika

AU - Rank, Christian

AU - Gotfredsen, Charlotte Held

AU - Larsen, T. O.

AU - Mortensen, Uffe Hasbro

PY - 2012

Y1 - 2012

N2 - With the aim of mapping the polyketome of A. nidulans we have made a library of strains, which individually overexpress PKS genes from an ectopic locus. A screen of this collection on different media demonstrated that AN6448 leads to production of 3-MOA. An inspection of the DNA sequence surrounding this gene uncovered a putative gene cluster including a gene, AN6446, with homology to transcription factors. Based on this observation, we decided to overexpress AN6446. A qRT-PCR analysis of this strain was used to delineate the borders of the gene cluster as well as to stimulate formation of cichorine and a number of new products, from the gene cluster. Subsequent deletion of all genes allowed several steps in the biosynthetic pathway in this cluster to be clarified.

AB - With the aim of mapping the polyketome of A. nidulans we have made a library of strains, which individually overexpress PKS genes from an ectopic locus. A screen of this collection on different media demonstrated that AN6448 leads to production of 3-MOA. An inspection of the DNA sequence surrounding this gene uncovered a putative gene cluster including a gene, AN6446, with homology to transcription factors. Based on this observation, we decided to overexpress AN6446. A qRT-PCR analysis of this strain was used to delineate the borders of the gene cluster as well as to stimulate formation of cichorine and a number of new products, from the gene cluster. Subsequent deletion of all genes allowed several steps in the biosynthetic pathway in this cluster to be clarified.

U2 - 10.1055/s-0032-1320546

DO - 10.1055/s-0032-1320546

M3 - Conference abstract in journal

SN - 0032-0943

VL - 78

SP - 1113

EP - 1113

JO - Planta Medica

JF - Planta Medica

IS - 11

T2 - International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF

Y2 - 28 July 2012 through 1 August 2012

ER -

Characterization of the AN6448 cluster in Aspergillus nidulans

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