With the aim of mapping the polyketome of A. nidulans we have made a library of strains, which individually overexpress PKS genes from an ectopic locus. A screen of this collection on different media demonstrated that AN6448 leads to production of 3-MOA. An inspection of the DNA sequence surrounding this gene uncovered a putative gene cluster including a gene, AN6446, with homology to transcription factors. Based on this observation, we decided to overexpress AN6446. A qRT-PCR analysis of this strain was used to delineate the borders of the gene cluster as well as to stimulate formation of cichorine and a number of new products, from the gene cluster. Subsequent deletion of all genes allowed several steps in the biosynthetic pathway in this cluster to be clarified.
|Conference||International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF|
|City||New York, NY|
|Period||28/07/2012 → 01/08/2012|