Characterization of the AN6448 cluster in Aspergillus nidulans

Jakob Blæsbjerg Nielsen, Marie Louise Klejnstrup, Paiman Khorsand-Jamal, Dorte Koefoed Holm, Michael Lynge Nielsen, Anna Monika Kabat, Christian Rank, Charlotte Held Gotfredsen, T. O. Larsen, Uffe Hasbro Mortensen

Research output: Contribution to journalConference abstract in journalResearchpeer-review

Abstract

With the aim of mapping the polyketome of A. nidulans we have made a library of strains, which individually overexpress PKS genes from an ectopic locus. A screen of this collection on different media demonstrated that AN6448 leads to production of 3-MOA. An inspection of the DNA sequence surrounding this gene uncovered a putative gene cluster including a gene, AN6446, with homology to transcription factors. Based on this observation, we decided to overexpress AN6446. A qRT-PCR analysis of this strain was used to delineate the borders of the gene cluster as well as to stimulate formation of cichorine and a number of new products, from the gene cluster. Subsequent deletion of all genes allowed several steps in the biosynthetic pathway in this cluster to be clarified.
Original languageEnglish
JournalPlanta Medica
Volume78
Issue number11
Pages (from-to)1113-1113
ISSN0032-0943
DOIs
Publication statusPublished - 2012
EventInternational Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF - New York, NY, United States
Duration: 28 Jul 20121 Aug 2012

Conference

ConferenceInternational Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF
CountryUnited States
CityNew York, NY
Period28/07/201201/08/2012

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