Characterization of nanomaterials in food by electron microscopy

Agnieszka Dudkiewicz, Karen Tiede, Katrin Löschner, Louise Helene Søgaard Jensen, Eric Jensen, Rafal Wierzbicki, Alistair B.A. Boxall, Kristian Mølhave

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Engineered nanomaterials (ENMs) are increasingly being used in the food industry. In order to assess the efficacy and the risks of these materials, it is essential to have access to methods that not only detect the nanomaterials, but also provide information on the characteristics of the materials (e.g., size and shape).This review presents an overview of electron microscopy (EM)-based methods that have been, or have the potential to be, applied to imaging ENMs in foodstuffs. We provide an overview of approaches to sample preparation, including drying, chemical treatment, fixation and cryogenic methods. We then describe standard and non-standard EM-based approaches that are available for imaging prepared samples. Finally, we present a strategy for selecting the most appropriate method for a particular foodstuff.
Original languageEnglish
JournalTrends in Analytical Chemistry
Volume30
Issue number1
Pages (from-to)28-43
ISSN0165-9936
DOIs
Publication statusPublished - 2011

Keywords

  • Sample preparation
  • Foodstuff; Imaging
  • Cryo-electron microscopy
  • Transmission electron microscopy (TEM)
  • Liquid electron microscopy
  • Scanning electron microscopy (SEM)
  • Nanoparticle
  • Detection
  • Environmental electron microscopy

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