Characterization of nanobiocomposite kappa-carrageenan film with Zataria multiflora essential oil and nanoclay

Saeedeh Shojaee-Aliabadi, Mohammad Amin Mohammadifar, Hedayat Hosseini, Abdorreza Mohammadi, Mehran Ghasemlou, Seyede Marzieh Hosseini, Mehrdad Haghshenas, Ramin Khaksar

Research output: Contribution to journalJournal articleResearchpeer-review


In this study, we aimed to improve the physical, mechanical and water-vapor permeability (WVP) properties of kappa-carrageenan (KC) films by including montmorillonite (MMT) nanoclay in the film-forming solution. To further improve these properties, the combined effect of Zataria multiflora Boiss essential oil (ZEO) and MMT was also investigated. The incorporation of MMT improved the physical and mechanical properties of KC film. Film made from KC alone had a tensile strength (TS) of 26.29 MPa, while the KC film with 10% nanoclay had a TS of 34.67. Further analysis was provided by X-ray diffraction and scanning electron microscopy that confirmed the dispersion of MMT in the KC matrix. It was also shown that the combined effect of nanoclay and ZEO significantly improved the TS and EB of KC films. ZEO decreased the WVP of the nanocomposite films; for example, 3% ZEO reduced WVP by around 78%. The antimicrobial activity of nanocomposite films was also studied using the overlay and vapor-phase methods; the films effectively inhibited the growth of five pathogens tested. Thus, the incorporation of both nanoclay and ZEO into KC films is a promising way to manufacture films with better mechanical, antimicrobial and WVP properties. (C) 2014 Elsevier B.V. All rights reserved.
Original languageEnglish
JournalInternational Journal of Biological Macromolecules
Pages (from-to)282-289
Number of pages8
Publication statusPublished - 2014
Externally publishedYes


  • Aluminum Silicates
  • Anti-Bacterial Agents
  • Carrageenan
  • Lamiaceae
  • Mechanical Processes
  • Nanocomposites
  • Oils, Volatile
  • Permeability
  • Volatilization
  • Water
  • clay
  • Antimicrobial activities
  • Edible film
  • Kappa-carrageenan
  • Nanobiocomposite
  • Zataria multiflora
  • Biochemistry
  • Molecular Biology
  • Structural Biology
  • Medicine (all)
  • carrageenan
  • essential oil
  • montmorillonite
  • nanoclay
  • nanocomposite
  • nanofilm
  • nanoparticle
  • unclassified drug
  • Zataria multiflora essential oil
  • aluminum silicate
  • antiinfective agent
  • water
  • antimicrobial activity
  • article
  • Bacillus cereus
  • concentration response
  • controlled study
  • dispersion
  • elongation at break
  • Escherichia coli
  • moisture
  • nanopharmaceutics
  • nonhuman
  • permeability
  • physical parameters
  • process optimization
  • Pseudomonas aeruginosa
  • Salmonella typhimurium
  • Staphylococcus aureus
  • tensile strength
  • thickness
  • water vapor permeability
  • chemistry
  • mechanics
  • volatilization
  • antimicrobial property
  • mechanical property
  • nanobiocomposite kappa-carrageenan film
  • nanoclay film
  • water-vapor permeability
  • Dicotyledones Angiospermae Spermatophyta Plantae (Angiosperms, Dicots, Plants, Spermatophytes, Vascular Plants) - Labiatae [26230] Zataria multiflora species
  • Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Endospore-forming Gram-Positives [07810] Bacillus cereus species pathogen, contaminant strain-PTCC 1154
  • Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Salmonella typhimurium species pathogen, contaminant strain-ATCC 14028 Escherichia coli species pathogen, contaminant strain-ATCC 25922
  • Gram-Negative Aerobic Rods and Cocci Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Pseudomonadaceae [06508] Pseudomonas aeruginosa species pathogen, contaminant strain-ATCC 27853
  • Gram-Positive Cocci Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Micrococcaceae [07702] Staphylococcus aureus species pathogen, contaminant strain-ATCC 25923
  • 10511, Biophysics - Bioengineering
  • 13502, Food technology - General and methods
  • 31000, Physiology and biochemistry of bacteria
  • scanning electron microscopy laboratory techniques, imaging and microscopy techniques
  • X-ray diffraction laboratory techniques, crystallographic techniques
  • Biomaterials
  • Foods


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