Characterization of Murine Intestinal Mesenchymal Stromal Cells

    Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

    Abstract

    The intestinal mucosa contains numerous and diverse subsets of immune cells that play an essential role in tissue homeostasis, immunity, and inflammation. Immune cells are diffusely distributed throughout the intestinal lamina propria (LP) within a network of mesenchymal stromal cells (MSCs) that are increasingly recognized as regulators of epithelial as well as immune cell development and function1,2. Despite this, our understanding of intestinal LP MSC ontogeny, heterogeneity, and function remains limited. To gain a better understanding of LP MSC heterogeneity, murine small intestinal and colon LP cell suspensions (after removal of the smooth muscle layer) were stained and flow cytometry sorted, removing epithelial (EPCAM+), endothelial (CD31+), lymphoid tissue associated (BP3+), hematopoietic (CD45+, Ter119+) and glial cells (NCAMhi). Single cell RNA-seq on sorted cells was subsequently performed by DROPseq. Our preliminary results suggest that the LP MSC compartment comprises numerous specialized subsets, a result we are currently confirming by flow cytometry and whole mount immunohistochemical analysis. Future studies aim to assess the ontogeny of these subsets and the potential role of each subset in intestinal immune homeostasis and disease.
    Original languageEnglish
    Publication date2018
    Publication statusPublished - 2018
    EventMucosal Immunology Course & Symposium 2018 - Oxford, United Kingdom
    Duration: 17 Jul 201820 Jul 2018
    Conference number: 2

    Conference

    ConferenceMucosal Immunology Course & Symposium 2018
    Number2
    CountryUnited Kingdom
    CityOxford
    Period17/07/201820/07/2018

    Cite this

    Pærregaard, S. I., Schussek, S., Jendholm, J., Niss, K., Vandamme, J., Sitnik, K., ... Agace, W. W. (2018). Characterization of Murine Intestinal Mesenchymal Stromal Cells. Abstract from Mucosal Immunology Course & Symposium 2018, Oxford, United Kingdom.
    Pærregaard, Simone Isling ; Schussek, Sophie ; Jendholm, Johan ; Niss, Kristoffer ; Vandamme, Julien ; Sitnik, Katarzyna ; Svensson-Frej, Marcus ; Agace, William W. / Characterization of Murine Intestinal Mesenchymal Stromal Cells. Abstract from Mucosal Immunology Course & Symposium 2018, Oxford, United Kingdom.
    @conference{a80b2238bd83441fa1ff22340c2011bc,
    title = "Characterization of Murine Intestinal Mesenchymal Stromal Cells",
    abstract = "The intestinal mucosa contains numerous and diverse subsets of immune cells that play an essential role in tissue homeostasis, immunity, and inflammation. Immune cells are diffusely distributed throughout the intestinal lamina propria (LP) within a network of mesenchymal stromal cells (MSCs) that are increasingly recognized as regulators of epithelial as well as immune cell development and function1,2. Despite this, our understanding of intestinal LP MSC ontogeny, heterogeneity, and function remains limited. To gain a better understanding of LP MSC heterogeneity, murine small intestinal and colon LP cell suspensions (after removal of the smooth muscle layer) were stained and flow cytometry sorted, removing epithelial (EPCAM+), endothelial (CD31+), lymphoid tissue associated (BP3+), hematopoietic (CD45+, Ter119+) and glial cells (NCAMhi). Single cell RNA-seq on sorted cells was subsequently performed by DROPseq. Our preliminary results suggest that the LP MSC compartment comprises numerous specialized subsets, a result we are currently confirming by flow cytometry and whole mount immunohistochemical analysis. Future studies aim to assess the ontogeny of these subsets and the potential role of each subset in intestinal immune homeostasis and disease.",
    author = "P{\ae}rregaard, {Simone Isling} and Sophie Schussek and Johan Jendholm and Kristoffer Niss and Julien Vandamme and Katarzyna Sitnik and Marcus Svensson-Frej and Agace, {William W.}",
    year = "2018",
    language = "English",
    note = "Mucosal Immunology Course & Symposium 2018, MICS 2018 ; Conference date: 17-07-2018 Through 20-07-2018",

    }

    Pærregaard, SI, Schussek, S, Jendholm, J, Niss, K, Vandamme, J, Sitnik, K, Svensson-Frej, M & Agace, WW 2018, 'Characterization of Murine Intestinal Mesenchymal Stromal Cells', Mucosal Immunology Course & Symposium 2018, Oxford, United Kingdom, 17/07/2018 - 20/07/2018.

    Characterization of Murine Intestinal Mesenchymal Stromal Cells. / Pærregaard, Simone Isling; Schussek, Sophie; Jendholm, Johan; Niss, Kristoffer; Vandamme, Julien; Sitnik, Katarzyna ; Svensson-Frej, Marcus; Agace, William W.

    2018. Abstract from Mucosal Immunology Course & Symposium 2018, Oxford, United Kingdom.

    Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

    TY - ABST

    T1 - Characterization of Murine Intestinal Mesenchymal Stromal Cells

    AU - Pærregaard, Simone Isling

    AU - Schussek, Sophie

    AU - Jendholm, Johan

    AU - Niss, Kristoffer

    AU - Vandamme, Julien

    AU - Sitnik, Katarzyna

    AU - Svensson-Frej, Marcus

    AU - Agace, William W.

    PY - 2018

    Y1 - 2018

    N2 - The intestinal mucosa contains numerous and diverse subsets of immune cells that play an essential role in tissue homeostasis, immunity, and inflammation. Immune cells are diffusely distributed throughout the intestinal lamina propria (LP) within a network of mesenchymal stromal cells (MSCs) that are increasingly recognized as regulators of epithelial as well as immune cell development and function1,2. Despite this, our understanding of intestinal LP MSC ontogeny, heterogeneity, and function remains limited. To gain a better understanding of LP MSC heterogeneity, murine small intestinal and colon LP cell suspensions (after removal of the smooth muscle layer) were stained and flow cytometry sorted, removing epithelial (EPCAM+), endothelial (CD31+), lymphoid tissue associated (BP3+), hematopoietic (CD45+, Ter119+) and glial cells (NCAMhi). Single cell RNA-seq on sorted cells was subsequently performed by DROPseq. Our preliminary results suggest that the LP MSC compartment comprises numerous specialized subsets, a result we are currently confirming by flow cytometry and whole mount immunohistochemical analysis. Future studies aim to assess the ontogeny of these subsets and the potential role of each subset in intestinal immune homeostasis and disease.

    AB - The intestinal mucosa contains numerous and diverse subsets of immune cells that play an essential role in tissue homeostasis, immunity, and inflammation. Immune cells are diffusely distributed throughout the intestinal lamina propria (LP) within a network of mesenchymal stromal cells (MSCs) that are increasingly recognized as regulators of epithelial as well as immune cell development and function1,2. Despite this, our understanding of intestinal LP MSC ontogeny, heterogeneity, and function remains limited. To gain a better understanding of LP MSC heterogeneity, murine small intestinal and colon LP cell suspensions (after removal of the smooth muscle layer) were stained and flow cytometry sorted, removing epithelial (EPCAM+), endothelial (CD31+), lymphoid tissue associated (BP3+), hematopoietic (CD45+, Ter119+) and glial cells (NCAMhi). Single cell RNA-seq on sorted cells was subsequently performed by DROPseq. Our preliminary results suggest that the LP MSC compartment comprises numerous specialized subsets, a result we are currently confirming by flow cytometry and whole mount immunohistochemical analysis. Future studies aim to assess the ontogeny of these subsets and the potential role of each subset in intestinal immune homeostasis and disease.

    M3 - Conference abstract for conference

    ER -

    Pærregaard SI, Schussek S, Jendholm J, Niss K, Vandamme J, Sitnik K et al. Characterization of Murine Intestinal Mesenchymal Stromal Cells. 2018. Abstract from Mucosal Immunology Course & Symposium 2018, Oxford, United Kingdom.