TY - JOUR
T1 - Characterization of isolates of Flavobacterium psychrophilum associated with coldwater disease or rainbow trout fry syndrome I : phenotypic and genomic studies
AU - Lorenzen, Ellen
AU - Dalsgaard, Inger
AU - Bernardet, Jean-Francois
PY - 1997
Y1 - 1997
N2 - Isolates of Flavobacterium psychrophilum (formerly Cytophaga psychrophila and Flexibacter psychrophilus) mainly originating from clinical outbreaks of either coldwater disease (CWD) or rainbow trout fry syndrome (RTFS) were studied for selected biochemical, physiological, morphological and genomic characteristics, and compared with previously characterized French and American strains. DNA hybridization studies showed that the Danish isolates were highly related to the type strain, F. psychrophilum NCIMB 1947(T). Plasmid profiling of Danish isolates and those from other European countries revealed differences, which might be related to differences in pathogenicity. European isolates originating from clinical outbreaks of either RTFS or CND usually harboured one plasmid of 3.2 kb, whereas isolates originating from fish with different or no disease signs had other profiles. Phenotypically, the Danish isolates appeared very homogeneous and shared most characteristics with the type strain, and with French and American strains studied by other authors. Further studies on the importance of the plasmids and the proteolytic activities of the bacterium might help in elucidating possible virulence factors.
AB - Isolates of Flavobacterium psychrophilum (formerly Cytophaga psychrophila and Flexibacter psychrophilus) mainly originating from clinical outbreaks of either coldwater disease (CWD) or rainbow trout fry syndrome (RTFS) were studied for selected biochemical, physiological, morphological and genomic characteristics, and compared with previously characterized French and American strains. DNA hybridization studies showed that the Danish isolates were highly related to the type strain, F. psychrophilum NCIMB 1947(T). Plasmid profiling of Danish isolates and those from other European countries revealed differences, which might be related to differences in pathogenicity. European isolates originating from clinical outbreaks of either RTFS or CND usually harboured one plasmid of 3.2 kb, whereas isolates originating from fish with different or no disease signs had other profiles. Phenotypically, the Danish isolates appeared very homogeneous and shared most characteristics with the type strain, and with French and American strains studied by other authors. Further studies on the importance of the plasmids and the proteolytic activities of the bacterium might help in elucidating possible virulence factors.
U2 - 10.3354/dao031197
DO - 10.3354/dao031197
M3 - Journal article
SN - 0177-5103
VL - 31
SP - 197
EP - 208
JO - Diseases of Aquatic Organisms
JF - Diseases of Aquatic Organisms
IS - 3
ER -