Characterization of Emericella nidulans RodA and DewA hydrophobin mutants

Britt Guillaume Jensen, Jakob Blæsbjerg Nielsen, Mona Højgaard Pedersen, Ib Søndergaard, Jens Christian Frisvad, Kristian Fog Nielsen

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    Abstract

    Hydrophobins are small amphiphilic proteins containing an eight cysteine pattern only found in filamentous fungi. They are involved in the attachment of hyphae to hydrophobic structures and the formation of aerial structures. Five Emericella nidulans mutant strains were examined to study the two hydrophobins RodA and DewA. Individual knock-out mutants rodAΔ, dewAΔ and the double deletion strain rodAΔdewAΔ were constructed. Furthermore, two strains containing a point mutation in the first of the cysteines of RodA (rodA-C57G), where one was coupled to the dewA deletion, were included. The reference strain (NID1) and dewAΔ displayed green conidia. However, rodAΔ and rodAΔdewAΔ showed a dark green/brown conidial pigmentation, while rodA-C57G and rodAC57G dewAΔ displayed lighter brown conidia. rodAΔ and rodAΔdewAΔ displayed a higher degree of hülle cells compared to the moderate amount observed for NID1 and dewAΔ, while rodA-C57G and rodA-C57G dewAΔ displayed a low number of hülle cells. NID1 and dewAΔ conidia were dispersed as spore chains. rodAΔ, rodAΔdewAΔ, rodA-C57G and rodA-C57G dewAΔ spores were associated in large clumps, where the conidia seemed to adhere to one another. The largest degree of spore clustering was observed for rodAΔ and rodA-C57G dewAΔ.
    Original languageEnglish
    Publication date2011
    Publication statusPublished - 2011
    EventInternational Aspergillus Meeting - Pacific Grove, California, USA
    Duration: 1 Jan 2011 → …
    Conference number: 8

    Conference

    ConferenceInternational Aspergillus Meeting
    Number8
    CityPacific Grove, California, USA
    Period01/01/2011 → …

    Cite this

    Jensen, B. G., Nielsen, J. B., Pedersen, M. H., Søndergaard, I., Frisvad, J. C., & Nielsen, K. F. (2011). Characterization of Emericella nidulans RodA and DewA hydrophobin mutants. Abstract from International Aspergillus Meeting, Pacific Grove, California, USA, .
    Jensen, Britt Guillaume ; Nielsen, Jakob Blæsbjerg ; Pedersen, Mona Højgaard ; Søndergaard, Ib ; Frisvad, Jens Christian ; Nielsen, Kristian Fog. / Characterization of Emericella nidulans RodA and DewA hydrophobin mutants. Abstract from International Aspergillus Meeting, Pacific Grove, California, USA, .
    @conference{a55052e9a34b4c0296b6d43923943c43,
    title = "Characterization of Emericella nidulans RodA and DewA hydrophobin mutants",
    abstract = "Hydrophobins are small amphiphilic proteins containing an eight cysteine pattern only found in filamentous fungi. They are involved in the attachment of hyphae to hydrophobic structures and the formation of aerial structures. Five Emericella nidulans mutant strains were examined to study the two hydrophobins RodA and DewA. Individual knock-out mutants rodAΔ, dewAΔ and the double deletion strain rodAΔdewAΔ were constructed. Furthermore, two strains containing a point mutation in the first of the cysteines of RodA (rodA-C57G), where one was coupled to the dewA deletion, were included. The reference strain (NID1) and dewAΔ displayed green conidia. However, rodAΔ and rodAΔdewAΔ showed a dark green/brown conidial pigmentation, while rodA-C57G and rodAC57G dewAΔ displayed lighter brown conidia. rodAΔ and rodAΔdewAΔ displayed a higher degree of h{\"u}lle cells compared to the moderate amount observed for NID1 and dewAΔ, while rodA-C57G and rodA-C57G dewAΔ displayed a low number of h{\"u}lle cells. NID1 and dewAΔ conidia were dispersed as spore chains. rodAΔ, rodAΔdewAΔ, rodA-C57G and rodA-C57G dewAΔ spores were associated in large clumps, where the conidia seemed to adhere to one another. The largest degree of spore clustering was observed for rodAΔ and rodA-C57G dewAΔ.",
    author = "Jensen, {Britt Guillaume} and Nielsen, {Jakob Bl{\ae}sbjerg} and Pedersen, {Mona H{\o}jgaard} and Ib S{\o}ndergaard and Frisvad, {Jens Christian} and Nielsen, {Kristian Fog}",
    year = "2011",
    language = "English",
    note = "International Aspergillus Meeting, ASPERFEST ; Conference date: 01-01-2011",

    }

    Jensen, BG, Nielsen, JB, Pedersen, MH, Søndergaard, I, Frisvad, JC & Nielsen, KF 2011, 'Characterization of Emericella nidulans RodA and DewA hydrophobin mutants', International Aspergillus Meeting, Pacific Grove, California, USA, 01/01/2011.

    Characterization of Emericella nidulans RodA and DewA hydrophobin mutants. / Jensen, Britt Guillaume; Nielsen, Jakob Blæsbjerg; Pedersen, Mona Højgaard; Søndergaard, Ib; Frisvad, Jens Christian; Nielsen, Kristian Fog.

    2011. Abstract from International Aspergillus Meeting, Pacific Grove, California, USA, .

    Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

    TY - ABST

    T1 - Characterization of Emericella nidulans RodA and DewA hydrophobin mutants

    AU - Jensen, Britt Guillaume

    AU - Nielsen, Jakob Blæsbjerg

    AU - Pedersen, Mona Højgaard

    AU - Søndergaard, Ib

    AU - Frisvad, Jens Christian

    AU - Nielsen, Kristian Fog

    PY - 2011

    Y1 - 2011

    N2 - Hydrophobins are small amphiphilic proteins containing an eight cysteine pattern only found in filamentous fungi. They are involved in the attachment of hyphae to hydrophobic structures and the formation of aerial structures. Five Emericella nidulans mutant strains were examined to study the two hydrophobins RodA and DewA. Individual knock-out mutants rodAΔ, dewAΔ and the double deletion strain rodAΔdewAΔ were constructed. Furthermore, two strains containing a point mutation in the first of the cysteines of RodA (rodA-C57G), where one was coupled to the dewA deletion, were included. The reference strain (NID1) and dewAΔ displayed green conidia. However, rodAΔ and rodAΔdewAΔ showed a dark green/brown conidial pigmentation, while rodA-C57G and rodAC57G dewAΔ displayed lighter brown conidia. rodAΔ and rodAΔdewAΔ displayed a higher degree of hülle cells compared to the moderate amount observed for NID1 and dewAΔ, while rodA-C57G and rodA-C57G dewAΔ displayed a low number of hülle cells. NID1 and dewAΔ conidia were dispersed as spore chains. rodAΔ, rodAΔdewAΔ, rodA-C57G and rodA-C57G dewAΔ spores were associated in large clumps, where the conidia seemed to adhere to one another. The largest degree of spore clustering was observed for rodAΔ and rodA-C57G dewAΔ.

    AB - Hydrophobins are small amphiphilic proteins containing an eight cysteine pattern only found in filamentous fungi. They are involved in the attachment of hyphae to hydrophobic structures and the formation of aerial structures. Five Emericella nidulans mutant strains were examined to study the two hydrophobins RodA and DewA. Individual knock-out mutants rodAΔ, dewAΔ and the double deletion strain rodAΔdewAΔ were constructed. Furthermore, two strains containing a point mutation in the first of the cysteines of RodA (rodA-C57G), where one was coupled to the dewA deletion, were included. The reference strain (NID1) and dewAΔ displayed green conidia. However, rodAΔ and rodAΔdewAΔ showed a dark green/brown conidial pigmentation, while rodA-C57G and rodAC57G dewAΔ displayed lighter brown conidia. rodAΔ and rodAΔdewAΔ displayed a higher degree of hülle cells compared to the moderate amount observed for NID1 and dewAΔ, while rodA-C57G and rodA-C57G dewAΔ displayed a low number of hülle cells. NID1 and dewAΔ conidia were dispersed as spore chains. rodAΔ, rodAΔdewAΔ, rodA-C57G and rodA-C57G dewAΔ spores were associated in large clumps, where the conidia seemed to adhere to one another. The largest degree of spore clustering was observed for rodAΔ and rodA-C57G dewAΔ.

    M3 - Conference abstract for conference

    ER -

    Jensen BG, Nielsen JB, Pedersen MH, Søndergaard I, Frisvad JC, Nielsen KF. Characterization of Emericella nidulans RodA and DewA hydrophobin mutants. 2011. Abstract from International Aspergillus Meeting, Pacific Grove, California, USA, .