Characterization of Emericella nidulans RodA and DewA hydrophobin mutants

Britt Guillaume Jensen, Jakob Blæsbjerg Nielsen, Mona Højgaard Pedersen, Ib Søndergaard, Jens Christian Frisvad, Kristian Fog Nielsen

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    Abstract

    Hydrophobins are small amphiphilic proteins containing an eight cysteine pattern only found in filamentous fungi. They are involved in the attachment of hyphae to hydrophobic structures and the formation of aerial structures. Five Emericella nidulans mutant strains were examined to study the two hydrophobins RodA and DewA. Individual knock-out mutants rodAΔ, dewAΔ and the double deletion strain rodAΔdewAΔ were constructed. Furthermore, two strains containing a point mutation in the first of the cysteines of RodA (rodA-C57G), where one was coupled to the dewA deletion, were included. The reference strain (NID1) and dewAΔ displayed green conidia. However, rodAΔ and rodAΔdewAΔ showed a dark green/brown conidial pigmentation, while rodA-C57G and rodAC57G dewAΔ displayed lighter brown conidia. rodAΔ and rodAΔdewAΔ displayed a higher degree of hülle cells compared to the moderate amount observed for NID1 and dewAΔ, while rodA-C57G and rodA-C57G dewAΔ displayed a low number of hülle cells. NID1 and dewAΔ conidia were dispersed as spore chains. rodAΔ, rodAΔdewAΔ, rodA-C57G and rodA-C57G dewAΔ spores were associated in large clumps, where the conidia seemed to adhere to one another. The largest degree of spore clustering was observed for rodAΔ and rodA-C57G dewAΔ.
    Original languageEnglish
    Publication date2011
    Publication statusPublished - 2011
    EventInternational Aspergillus Meeting - Pacific Grove, California, USA
    Duration: 1 Jan 2011 → …
    Conference number: 8

    Conference

    ConferenceInternational Aspergillus Meeting
    Number8
    CityPacific Grove, California, USA
    Period01/01/2011 → …

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