Characterization of Diarrheagenic Enteroaggregative Escherichia coli in Danish Adults-Antibiotic Treatment Does Not Reduce Duration of Diarrhea

Research output: Contribution to journalJournal article – Annual report year: 2018Researchpeer-review



  • Author: Hebbelstrup Jensen, Betina

    Statens Serum Institut

  • Author: Adler Sørensen, Camilla

    Statens Serum Institut

  • Author: Hebbelstrup Rye Rasmussen, Stig

    University of Southern Denmark

  • Author: Rejkjær Holm, Dorthe

    Statens Serum Institut

  • Author: Friis-Møller, Alice

    University of Copenhagen

  • Author: Engberg, Jørgen

    University of Copenhagen

  • Author: Mirsepasi-Lauridsen, Hengameh Chloé

    Statens Serum Institut

  • Author: Struve, Carsten

    Statens Serum Institut, Denmark

  • Author: Hammerum, Anette M.

    Statens Serum Institut, Denmark

  • Author: Porsbo, Lone Jannok

    National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, 2860, Søborg, Denmark

  • Author: Petersen, Randi Føns

    Statens Serum Institut, Denmark

  • Author: Petersen, Andreas Munk

    University of Copenhagen

  • Author: Krogfelt, Karen Angeliki

    Statens Serum Institut, Denmark

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Enteroaggregative Escherichia coli (EAEC) is frequently isolated from sporadic cases of diarrhea and in outbreaks of gastroenteritis in several regions of the world. The pathophysiology of EAEC continues to be enigmatic, and the efficacy of antibiotic treatment in EAEC-associated diarrhea has been discussed. Since the level of antibiotic resistance is increasing, it is essential to restrict the use of antibiotics to prevent further resistance development. We aimed to investigate EAEC strains in adult Danish patients suffering from diarrhea and from healthy controls. We examined the antibiotic resistance in EAEC strains, the clinical response to antibiotic treatment in EAEC diarrheal cases, and the distribution of virulence genes in diarrheal cases. The EAEC strains were collected from patients suffering from diarrhea in a Danish multicenter study. A medical doctor interviewed the patients by using a questionnaire regarding gastrointestinal symptoms, exposures, and use of antibiotic and over-the-counter antidiarrheal drugs. Follow-up was performed after 3-5 months to inquire about differential diagnosis to gastrointestinal disease. A multiplex polymerase chain reaction characterized virulence genes in diarrheal cases. Finally, the level of antibiotic resistance was examined by using the disc diffusion method. Asymptomatic carriage of EAEC in the adult Danish population was rare, in contrast to findings in healthy Danish children. The duration of diarrhea was not shortened by antibiotic treatment, specifically ciprofloxacin treatment, or by over-the-counter antidiarrheal drugs. Follow-up revealed no pathology in diarrheal patients apart from irritable bowel syndrome in two patients. A high number of patients suffered from long-term diarrhea, which was associated with the enterotoxin EAST-1 and a high virulence factor score. A high level of antibiotic resistance was observed and 58% of the EAEC strains were multidrug resistant. Multidrug resistance was most pronounced in cases of travelers' diarrhea, and it was seen that antibiotic treatment did not reduce the duration of diarrhea.

Original languageEnglish
Article number306
JournalFrontiers in Cellular and Infection Microbiology
Number of pages12
Publication statusPublished - 1 Jan 2018
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • antibiotic resistance, diarrhea, EAEC, Enteroaggregative Escherichia coli, multidrug resistance

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