Characterization of Campylobacter jejuni applying flaA short variable region sequencing, multilocus sequencing and Fourier transform infrared spectroscopy

Mathilde Hartmann Josefsen, Lise Bonnichsen, Jonas Larsson, Eva Møller Nielsen, Martina Fricker, Monica Ehling-Schulz, Jeffrey Hoorfar

Research output: Contribution to conferencePosterResearch

Abstract

In the search for effective control and intervention strategies for foodborne Campylobacter, the need for a reliable characterization method remains. Understanding the dynamics of infection and the epidemiology of Campylobacter in the primary production is a corner stone in this context. This study describes the evaluation of a set of characterization methods on a coherent strain collection, with a high isolate to sample ratio, containing both epidemiologically related and unrelated strains. To evaluate the strain discriminatory potential of characterization methods for Campylobacter jejuni, genetic flaA short variable region sequencing and phenetic Fourier transform infrared (FTIR) spectroscopy was applied on a collection of 102 Campylobacter jejuni isolated from continuous sampling of organic, free range geese and chickens. FTIR has been shown to serve as a valuable tool in typing of thermotolerant Campylobacter. A subset of isolates, initially discriminated by flaA sequencing, was further subjected to multilocus sequence typing (MLST). The findings of this study revealed flaA sequencing to have a slightly higher discriminatory power than FTIR indicated by a Simpsons diversity index of 0.94 and 0.92, respectively. Dendrograms based on cluster analysis grouped the isolates in 6 clusters based on flaA sequences and 9 clusters based on the FTIR spectroscopy profiles. Furthermore, the cluster analysis of flaA sequences, MLST and FTIR spectroscopy profiles showed a surprisingly high degree of congruence, assigning the isolates to similar cluster structures.
Original languageEnglish
Publication date2010
Publication statusPublished - 2010
Event22nd International ICFMH Symposium - Copenhagen, Denmark
Duration: 30 Aug 20103 Sep 2010
Conference number: 22

Conference

Conference22nd International ICFMH Symposium
Number22
CountryDenmark
CityCopenhagen
Period30/08/201003/09/2010

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