Characterization of CA-MRSA TCH1516 exposed to nafcillin in bacteriological and physiological media

Saugat Poudel, Hannah Tsunemoto, Michael Meehan, Richard Szubin, Connor A. Olson, Anne Lamsa, Yara Seif, Nicholas Dillon, Alison Vrbanac, Joseph Sugie, Samira Dahesh, Jonathan M. Monk, Pieter C. Dorrestein, Joseph Pogliano, Rob Knight, Victor Nizet, Bernhard O. Palsson, Adam M. Feist

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Abstract

Cation adjusted-Mueller Hinton Broth (CA-MHB) is the standard bacteriological medium utilized in the clinic for the determination of antibiotic susceptibility. However, a growing number of literature has demonstrated that media conditions can cause a substantial difference in the efficacy of antibiotics and antimicrobials. Recent studies have also shown that minimum inhibitory concentration (MIC) tests performed in standard cell culture media (e.g. RPMI and DMEM) are more indicative of in vivo antibiotic efficacy, presumably because they are a better proxy for the human host's physiological conditions. The basis for the bacterial media dependent susceptibility to antibiotics remains undefined. To address this question, we characterized the physiological response of methicillin-resistant Staphylococcus aureus (MRSA) during exposure to sub-inhibitory concentrations of the beta-lactam antibiotic nafcillin in either CA-MHB or RPMI + 10% LB (R10LB). Here, we present high quality transcriptomic, exo-metabolomic and morphological data paired with growth and susceptibility results for MRSA cultured in either standard bacteriologic or more physiologic relevant medium.
Original languageEnglish
Article number43
JournalScientific Data
Volume6
Issue number1
ISSN2052-4463
DOIs
Publication statusPublished - 2019

Cite this

Poudel, Saugat ; Tsunemoto, Hannah ; Meehan, Michael ; Szubin, Richard ; Olson, Connor A. ; Lamsa, Anne ; Seif, Yara ; Dillon, Nicholas ; Vrbanac, Alison ; Sugie, Joseph ; Dahesh, Samira ; Monk, Jonathan M. ; Dorrestein, Pieter C. ; Pogliano, Joseph ; Knight, Rob ; Nizet, Victor ; Palsson, Bernhard O. ; Feist, Adam M. / Characterization of CA-MRSA TCH1516 exposed to nafcillin in bacteriological and physiological media. In: Scientific Data. 2019 ; Vol. 6, No. 1.
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title = "Characterization of CA-MRSA TCH1516 exposed to nafcillin in bacteriological and physiological media",
abstract = "Cation adjusted-Mueller Hinton Broth (CA-MHB) is the standard bacteriological medium utilized in the clinic for the determination of antibiotic susceptibility. However, a growing number of literature has demonstrated that media conditions can cause a substantial difference in the efficacy of antibiotics and antimicrobials. Recent studies have also shown that minimum inhibitory concentration (MIC) tests performed in standard cell culture media (e.g. RPMI and DMEM) are more indicative of in vivo antibiotic efficacy, presumably because they are a better proxy for the human host's physiological conditions. The basis for the bacterial media dependent susceptibility to antibiotics remains undefined. To address this question, we characterized the physiological response of methicillin-resistant Staphylococcus aureus (MRSA) during exposure to sub-inhibitory concentrations of the beta-lactam antibiotic nafcillin in either CA-MHB or RPMI + 10{\%} LB (R10LB). Here, we present high quality transcriptomic, exo-metabolomic and morphological data paired with growth and susceptibility results for MRSA cultured in either standard bacteriologic or more physiologic relevant medium.",
author = "Saugat Poudel and Hannah Tsunemoto and Michael Meehan and Richard Szubin and Olson, {Connor A.} and Anne Lamsa and Yara Seif and Nicholas Dillon and Alison Vrbanac and Joseph Sugie and Samira Dahesh and Monk, {Jonathan M.} and Dorrestein, {Pieter C.} and Joseph Pogliano and Rob Knight and Victor Nizet and Palsson, {Bernhard O.} and Feist, {Adam M.}",
year = "2019",
doi = "10.1038/s41597-019-0051-4",
language = "English",
volume = "6",
journal = "Scientific Data",
issn = "2052-4463",
publisher = "Nature Publishing Group",
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Poudel, S, Tsunemoto, H, Meehan, M, Szubin, R, Olson, CA, Lamsa, A, Seif, Y, Dillon, N, Vrbanac, A, Sugie, J, Dahesh, S, Monk, JM, Dorrestein, PC, Pogliano, J, Knight, R, Nizet, V, Palsson, BO & Feist, AM 2019, 'Characterization of CA-MRSA TCH1516 exposed to nafcillin in bacteriological and physiological media', Scientific Data, vol. 6, no. 1, 43. https://doi.org/10.1038/s41597-019-0051-4

Characterization of CA-MRSA TCH1516 exposed to nafcillin in bacteriological and physiological media. / Poudel, Saugat; Tsunemoto, Hannah; Meehan, Michael; Szubin, Richard; Olson, Connor A.; Lamsa, Anne; Seif, Yara; Dillon, Nicholas; Vrbanac, Alison; Sugie, Joseph; Dahesh, Samira; Monk, Jonathan M.; Dorrestein, Pieter C.; Pogliano, Joseph; Knight, Rob; Nizet, Victor; Palsson, Bernhard O.; Feist, Adam M.

In: Scientific Data, Vol. 6, No. 1, 43, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Characterization of CA-MRSA TCH1516 exposed to nafcillin in bacteriological and physiological media

AU - Poudel, Saugat

AU - Tsunemoto, Hannah

AU - Meehan, Michael

AU - Szubin, Richard

AU - Olson, Connor A.

AU - Lamsa, Anne

AU - Seif, Yara

AU - Dillon, Nicholas

AU - Vrbanac, Alison

AU - Sugie, Joseph

AU - Dahesh, Samira

AU - Monk, Jonathan M.

AU - Dorrestein, Pieter C.

AU - Pogliano, Joseph

AU - Knight, Rob

AU - Nizet, Victor

AU - Palsson, Bernhard O.

AU - Feist, Adam M.

PY - 2019

Y1 - 2019

N2 - Cation adjusted-Mueller Hinton Broth (CA-MHB) is the standard bacteriological medium utilized in the clinic for the determination of antibiotic susceptibility. However, a growing number of literature has demonstrated that media conditions can cause a substantial difference in the efficacy of antibiotics and antimicrobials. Recent studies have also shown that minimum inhibitory concentration (MIC) tests performed in standard cell culture media (e.g. RPMI and DMEM) are more indicative of in vivo antibiotic efficacy, presumably because they are a better proxy for the human host's physiological conditions. The basis for the bacterial media dependent susceptibility to antibiotics remains undefined. To address this question, we characterized the physiological response of methicillin-resistant Staphylococcus aureus (MRSA) during exposure to sub-inhibitory concentrations of the beta-lactam antibiotic nafcillin in either CA-MHB or RPMI + 10% LB (R10LB). Here, we present high quality transcriptomic, exo-metabolomic and morphological data paired with growth and susceptibility results for MRSA cultured in either standard bacteriologic or more physiologic relevant medium.

AB - Cation adjusted-Mueller Hinton Broth (CA-MHB) is the standard bacteriological medium utilized in the clinic for the determination of antibiotic susceptibility. However, a growing number of literature has demonstrated that media conditions can cause a substantial difference in the efficacy of antibiotics and antimicrobials. Recent studies have also shown that minimum inhibitory concentration (MIC) tests performed in standard cell culture media (e.g. RPMI and DMEM) are more indicative of in vivo antibiotic efficacy, presumably because they are a better proxy for the human host's physiological conditions. The basis for the bacterial media dependent susceptibility to antibiotics remains undefined. To address this question, we characterized the physiological response of methicillin-resistant Staphylococcus aureus (MRSA) during exposure to sub-inhibitory concentrations of the beta-lactam antibiotic nafcillin in either CA-MHB or RPMI + 10% LB (R10LB). Here, we present high quality transcriptomic, exo-metabolomic and morphological data paired with growth and susceptibility results for MRSA cultured in either standard bacteriologic or more physiologic relevant medium.

U2 - 10.1038/s41597-019-0051-4

DO - 10.1038/s41597-019-0051-4

M3 - Journal article

VL - 6

JO - Scientific Data

JF - Scientific Data

SN - 2052-4463

IS - 1

M1 - 43

ER -