Characterization of β-glucosidase from a strain of Penicillium purpurogenum KJS506

Marimuthu Jeya, Ah-Reum Joo, Kyoung-Mi Lee, Manish Kumar Tiwari, Kyoung-Min Lee, Sang-Hwan Kim, Jung-Kul Lee

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A novel β-glucosidase (BGL)-producing strain was isolated and identified as Penicillium purpurogenum KJS506 based on its morphology and internal transcribed spacer (ITS) rDNA gene sequence. When rice straw and corn steep powder were used as carbon and nitrogen sources, respectively, the maximal BGL activity of 12.3 U ml−1, one of the highest levels among BGL-producing microorganisms was observed. The optimum temperature and pH for BGL production were 32 °C and 4, respectively. An extracellular BGL was purified to homogeneity by sequential chromatography of P. purpurogenum culture supernatants, and the purified BGL showed higher activity (Vmax=934 U mg protein–1) than most BGLs from other sources. The complete ORF of bgl3 was cloned from P. purpurogenum by a modified thermal asymmetric interlaced polymerase chain reaction. The bgl3 gene consists of a 2,571-bp ORF and encodes a putative protein containing 856 amino acids with a calculated molecular mass of 89,624 Da. The putative gene product was identified as a member of glycoside hydrolase family 3. The present results should contribute to improved industrial production of BGL by P. purpurogenum KJS506.
Original languageEnglish
JournalApplied Microbiology and Biotechnology
Volume86
Issue number5
Pages (from-to)1473–1484
ISSN0175-7598
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • Cloning
  • β-Glucosidase
  • Homology modeling
  • Penicillium purpurogenum
  • TAIL-PCR

Cite this

Jeya, M., Joo, A-R., Lee, K-M., Tiwari, M. K., Lee, K-M., Kim, S-H., & Lee, J-K. (2010). Characterization of β-glucosidase from a strain of Penicillium purpurogenum KJS506. Applied Microbiology and Biotechnology, 86(5), 1473–1484. https://doi.org/10.1007/s00253-009-2395-8