Characterization and identification of lactic acid bacteria in "morcilla de Burgos"

EM Santos, I. Jaime, J. Rovira, Ulrike Lyhs, H. Korkeala, J. Bjorkroth

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A total of 176 lactic acid bacteria (LAB) isolated from a typical Spanish blood sausage called "morcilla de Burgos" were identified by means of phenotypic characteristics and 16S rDNA RFLP (ribotyping). LAB were isolated from "morcilla" of different producers and in different storage periods, which includes unpackaged, vacuum and modified atmosphere packaged "morcilla" and vacuum packed and pasteurised "morcilla". The knowledge of specific spoilage bacteria of "morcilla de Burgos" will be useful to design new preservation methods to extend the shelf-life of this product. Identification made according to phenotypic and biochemical characteristics shows the majority of the isolates were heterofermentative LAB (93.2%) and eight different bacterial groups could be distinguished (A-G). Weisella viridescens was the main species detected (42%). In addition, Leuconostoc spp. (23.9%), Weissella confusa (11.4%) and Lactobacillus fructosus (5.7%) species were found. Few strains were phenotypically missidentified as Lactobacillus sanfrancisco, Pediococcus spp., Lactobacillus sakei/curvatus and Carnobacterium spp. and 11 strains remained unknown. Most of the leuconostocs were identified as Leuconostoc mesenteroides and Leuconostoc carnosum species. Ribotyping shows a quite good correlation with phenotypic methods, although it has been possible to identify 15 different clusters. W. viridescens and leuconostocs were also the predominant LAB. Strains identified as W confusa by phenotypic characteristics were resolved in W confusa and Weissella cibaria by ribotyping. Neither Carnobacterium piscicola nor Lb. sanfrancisco were identified by means of genotypic method. All Lb. fructosus strains and some more included in different phenotypic groups (17 strains in total) could not be associated with any reference strain (cluster VII). (C) 2004 Elsevier B.V. All rights reserved.
Original languageEnglish
JournalInternational Journal of Food Microbiology
Volume97
Issue number3
Pages (from-to)285-296
ISSN0168-1605
DOIs
Publication statusPublished - 2005
Externally publishedYes

Keywords

  • Food Science
  • Microbiology
  • Blood sausages
  • Identification
  • Lactic acid bacteria
  • Ribotyping
  • Spoilage
  • DNA 16S
  • article
  • bacterial strain
  • bacterium identification
  • bacterium isolation
  • Carnobacterium
  • controlled study
  • food packaging
  • food spoilage
  • food storage
  • lactic acid bacterium
  • Lactobacillus curvatus
  • lactobacillus fructosus
  • lactobacillus sanfranscisco
  • Leuconostoc
  • meat
  • nonhuman
  • phenotype
  • restriction fragment length polymorphism
  • ribotyping
  • Weisella confusa
  • weisella viridescens
  • Animals
  • DNA, Bacterial
  • Food Handling
  • Food Microbiology
  • Food Packaging
  • Food Preservation
  • Lactobacillus
  • Meat Products
  • Phylogeny
  • Swine
  • Bacteria (microorganisms)
  • Carnobacterium maltaromaticum
  • Lactobacillus sakei
  • Lactobacillus sanfranciscensis
  • Leuconostoc carnosum
  • Leuconostoc fructosum
  • Leuconostoc mesenteroides
  • Moorella group
  • Pediococcus
  • Piscicola
  • Weissella cibaria
  • Weissella confusa
  • Weissella viridescens
  • FOOD
  • MICROBIOLOGY
  • PACKAGED VIENNA SAUSAGES
  • MEAT-PRODUCTS
  • LACTOBACILLUS-VIRIDESCENS
  • LEUCONOSTOC-CARNOSUM
  • FERMENTED SAUSAGES
  • DNA HYBRIDIZATION
  • ROPY SLIME
  • SP NOV.
  • SPOILAGE
  • STRAINS
  • lactic acid bacteria
  • identification
  • blood sausages
  • spoilage
  • bacterial identification
  • morcilla de Burgos
  • sausage meat product
  • Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Gram-Positive Cocci [07700] Leuconostoc genus contaminant, pathogen Leuconostoc carnosum species contaminant, pathogen Leuconostoc mesenteroides species contaminant, pathogen Pediococcus genus contaminant, pathogen Weissella confusa species contaminant, pathogen Weissella viridescens species contaminant, pathogen
  • Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Regular Nonsporing Gram-Positive Rods [07830] Carnobacterium genus contaminant, pathogen Lactobacillus curvatus species contaminant, pathogen Lactobacillus fructosus species contaminant, pathogen Lactobacillus sakei species contaminant, pathogen Lactobacillus sanfrancisco species contaminant, pathogen
  • Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteria [05000] lactic acid bacteria common contaminant, pathogen
  • 16S rDNA 16S ribosomal DNA
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 13502, Food technology - General and methods
  • 13516, Food technology - Meats and meat by-products
  • 31000, Physiology and biochemistry of bacteria
  • phenotyping genetic techniques, laboratory techniques
  • RFLP restriction fragment length polymorphism genetic techniques, laboratory techniques
  • ribotyping genetic techniques, laboratory techniques
  • Foods
  • Infection
  • BACTERIA
  • BLOOD SAUSAGES
  • GENETIC TECHNIQUES
  • LACTIC ACID BACTERIA
  • MICROBIOLOGICAL TECHNIQUES
  • RIBOTYPING
  • SAUSAGES
  • Meat, poultry and game
  • Sausages (including casings)

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