Characterisation of the gut microflora in rainbow trout fry (Oncorhynchus mykiss) using deep-sequencing

Hans-Christian Ingerslev, Inger Dalsgaard, Mette Boye, Lone Madsen

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

    Abstract

    For many years it has been known that the bacterial microflora in the gut of warm-blooded animals exists in harmony with the host and exert various beneficial effects on the health by their metabolic activities. Hence, the gut microbiota has a high importance for the animal. In many studies from e.g. humans and the pig mapping of the bacterial flora from the gut have shown dominance by some specific bacterial groups, and this bacterial profile is termed as a ‘core microbiota’. For lower vertebrates like fish mapping of the bacterial flora in the gastrointestinal system is to date a relatively new research field and previous studies have mainly been done on bacterial species that can be cultured or by classical molecular techniques like T-RFLP and DGGE. In the last recent years deep-sequencing techniques have enabled sequencing and mapping of entire microbial communities from for instance an environmental sample or a tissue / fecal sample from an animal. These metagenomic studies have provided new and deeper insight into the microbial ecology and the influence of the microbiota in warm-blooded animals.
    In this study next-generation sequencing of the 16S rDNA gene on the Illumina HiSeq platform was used to examine the composition of the microbial flora in the gut of rainbow trout (Oncorhynchus mykiss) fry. The fish were examined before and after first-feeding and after administration by commercial probiotic lactobacilli to the feed.
    Original languageEnglish
    Title of host publicationDAFINET Workshop : Book of abstracts
    Number of pages1
    PublisherDanish Fish Immunology Research Centre and Network
    Publication date2012
    Publication statusPublished - 2012
    EventDAFINET Workshop: The Ontogeny of the Fish Immune System - Copenhagen, Denmark
    Duration: 24 Apr 201225 Apr 2012
    http://www.dafinet.dk

    Conference

    ConferenceDAFINET Workshop
    CountryDenmark
    CityCopenhagen
    Period24/04/201225/04/2012
    Internet address

    Cite this

    Ingerslev, H-C., Dalsgaard, I., Boye, M., & Madsen, L. (2012). Characterisation of the gut microflora in rainbow trout fry (Oncorhynchus mykiss) using deep-sequencing. In DAFINET Workshop: Book of abstracts Danish Fish Immunology Research Centre and Network.
    Ingerslev, Hans-Christian ; Dalsgaard, Inger ; Boye, Mette ; Madsen, Lone. / Characterisation of the gut microflora in rainbow trout fry (Oncorhynchus mykiss) using deep-sequencing. DAFINET Workshop: Book of abstracts. Danish Fish Immunology Research Centre and Network, 2012.
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    title = "Characterisation of the gut microflora in rainbow trout fry (Oncorhynchus mykiss) using deep-sequencing",
    abstract = "For many years it has been known that the bacterial microflora in the gut of warm-blooded animals exists in harmony with the host and exert various beneficial effects on the health by their metabolic activities. Hence, the gut microbiota has a high importance for the animal. In many studies from e.g. humans and the pig mapping of the bacterial flora from the gut have shown dominance by some specific bacterial groups, and this bacterial profile is termed as a ‘core microbiota’. For lower vertebrates like fish mapping of the bacterial flora in the gastrointestinal system is to date a relatively new research field and previous studies have mainly been done on bacterial species that can be cultured or by classical molecular techniques like T-RFLP and DGGE. In the last recent years deep-sequencing techniques have enabled sequencing and mapping of entire microbial communities from for instance an environmental sample or a tissue / fecal sample from an animal. These metagenomic studies have provided new and deeper insight into the microbial ecology and the influence of the microbiota in warm-blooded animals.In this study next-generation sequencing of the 16S rDNA gene on the Illumina HiSeq platform was used to examine the composition of the microbial flora in the gut of rainbow trout (Oncorhynchus mykiss) fry. The fish were examined before and after first-feeding and after administration by commercial probiotic lactobacilli to the feed.",
    author = "Hans-Christian Ingerslev and Inger Dalsgaard and Mette Boye and Lone Madsen",
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    Ingerslev, H-C, Dalsgaard, I, Boye, M & Madsen, L 2012, Characterisation of the gut microflora in rainbow trout fry (Oncorhynchus mykiss) using deep-sequencing. in DAFINET Workshop: Book of abstracts. Danish Fish Immunology Research Centre and Network, DAFINET Workshop, Copenhagen, Denmark, 24/04/2012.

    Characterisation of the gut microflora in rainbow trout fry (Oncorhynchus mykiss) using deep-sequencing. / Ingerslev, Hans-Christian; Dalsgaard, Inger; Boye, Mette; Madsen, Lone.

    DAFINET Workshop: Book of abstracts. Danish Fish Immunology Research Centre and Network, 2012.

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

    TY - ABST

    T1 - Characterisation of the gut microflora in rainbow trout fry (Oncorhynchus mykiss) using deep-sequencing

    AU - Ingerslev, Hans-Christian

    AU - Dalsgaard, Inger

    AU - Boye, Mette

    AU - Madsen, Lone

    PY - 2012

    Y1 - 2012

    N2 - For many years it has been known that the bacterial microflora in the gut of warm-blooded animals exists in harmony with the host and exert various beneficial effects on the health by their metabolic activities. Hence, the gut microbiota has a high importance for the animal. In many studies from e.g. humans and the pig mapping of the bacterial flora from the gut have shown dominance by some specific bacterial groups, and this bacterial profile is termed as a ‘core microbiota’. For lower vertebrates like fish mapping of the bacterial flora in the gastrointestinal system is to date a relatively new research field and previous studies have mainly been done on bacterial species that can be cultured or by classical molecular techniques like T-RFLP and DGGE. In the last recent years deep-sequencing techniques have enabled sequencing and mapping of entire microbial communities from for instance an environmental sample or a tissue / fecal sample from an animal. These metagenomic studies have provided new and deeper insight into the microbial ecology and the influence of the microbiota in warm-blooded animals.In this study next-generation sequencing of the 16S rDNA gene on the Illumina HiSeq platform was used to examine the composition of the microbial flora in the gut of rainbow trout (Oncorhynchus mykiss) fry. The fish were examined before and after first-feeding and after administration by commercial probiotic lactobacilli to the feed.

    AB - For many years it has been known that the bacterial microflora in the gut of warm-blooded animals exists in harmony with the host and exert various beneficial effects on the health by their metabolic activities. Hence, the gut microbiota has a high importance for the animal. In many studies from e.g. humans and the pig mapping of the bacterial flora from the gut have shown dominance by some specific bacterial groups, and this bacterial profile is termed as a ‘core microbiota’. For lower vertebrates like fish mapping of the bacterial flora in the gastrointestinal system is to date a relatively new research field and previous studies have mainly been done on bacterial species that can be cultured or by classical molecular techniques like T-RFLP and DGGE. In the last recent years deep-sequencing techniques have enabled sequencing and mapping of entire microbial communities from for instance an environmental sample or a tissue / fecal sample from an animal. These metagenomic studies have provided new and deeper insight into the microbial ecology and the influence of the microbiota in warm-blooded animals.In this study next-generation sequencing of the 16S rDNA gene on the Illumina HiSeq platform was used to examine the composition of the microbial flora in the gut of rainbow trout (Oncorhynchus mykiss) fry. The fish were examined before and after first-feeding and after administration by commercial probiotic lactobacilli to the feed.

    M3 - Conference abstract in proceedings

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    PB - Danish Fish Immunology Research Centre and Network

    ER -

    Ingerslev H-C, Dalsgaard I, Boye M, Madsen L. Characterisation of the gut microflora in rainbow trout fry (Oncorhynchus mykiss) using deep-sequencing. In DAFINET Workshop: Book of abstracts. Danish Fish Immunology Research Centre and Network. 2012