Characterisation of lactic acid bacteria from spoiled, vacuum-packaged, cold-smoked rainbow trout using ribotyping

Ulrike Lyhs, J. Bjorkroth, H. Korkeala

Research output: Contribution to journalJournal articleResearchpeer-review

152 Downloads (Pure)

Abstract

A total of 405 lactic acid bacteria (LAB) isolated from spoiled, vacuum-packaged, salted, sodium nitrite- or potassium nitrate-treated, cold-smoked rainbow trout stored at 4 degrees C or 8 degrees C were characterised and identified using a molecular method. The isolates were initially classified according to their restriction endonuclease profiles using HindIII and EcoRI restriction endonucleases and further characterised by rRNA gene restriction patterns (ribotypes). Numerical analysis of these ribopatterns was performed together with 19 reference LAB strain patterns in order to identify the isolates to species level. The strains were divided with HindIII and EcoRI ribopatterns into ten and nine clusters at the similarity level of 65% and 50%, respectively. The Leuconostoc-clusters and the Lb. sakei/Lb. curvatus-clusters formed the two main groups. Only one isolate was identified as Lactobacillus plantarum and no Carnobacterium strains were discovered. For both enzymes, the 35 isolates possessing six individual ribotypes and forming five clusters could not be identified further with the reference strains used. The relative proportion of Leuconostoc mesenteroides subsp. mesenteroides was higher in all samples stored at 4 degrees C. Most of the Leuconostoc citreum were found in the samples stored at 8 degrees C, and particularly in the nitrite-treated samples. (C) 1999 Elsevier Science B.V. All rights reserved.
Original languageEnglish
JournalInternational Journal of Food Microbiology
Volume52
Issue number1-2
Pages (from-to)77-84
ISSN0168-1605
DOIs
Publication statusPublished - 1999
Externally publishedYes

Keywords

  • Food Science
  • Microbiology
  • Fish
  • Lactic acid bacteria
  • Restriction endonuclease analysis
  • Ribotyping
  • Spoilage
  • Vacuum-packaging
  • potassium nitrate
  • restriction endonuclease
  • sodium nitrite
  • animal tissue
  • article
  • carnobacterium
  • food packaging
  • lactic acid bacterium
  • lactobacillus
  • lactobacillus plantarum
  • leuconostoc
  • nonhuman
  • rainbow trout
  • ribotyping
  • sea food
  • vacuum
  • Animals
  • Blotting, Southern
  • Cluster Analysis
  • Deoxyribonuclease HindIII
  • Deoxyribonucleases, Type II Site-Specific
  • DNA Probes
  • DNA, Bacterial
  • Food Microbiology
  • Food Packaging
  • Food Preservation
  • Image Processing, Computer-Assisted
  • Lactobacillus
  • Leuconostoc
  • Oncorhynchus mykiss
  • Phylogeny
  • RNA, Ribosomal
  • Vacuum
  • Carnobacterium
  • Lactobacillus plantarum
  • Leuconostoc citreum
  • Leuconostoc mesenteroides subsp. mesenteroides
  • Salmonidae
  • EC 3.1.21.- Deoxyribonuclease HindIII
  • EC 3.1.21.4 CCWGG-specific type II deoxyribonucleases
  • EC 3.1.21.4 Deoxyribonucleases, Type II Site-Specific
  • FOOD
  • MICROBIOLOGY
  • LACTOBACILLUS-SAKE KATAGIRI
  • CURVATUS ABO-ELNAGA
  • EMENDED DESCRIPTIONS
  • ROPY SLIME
  • IDENTIFICATION
  • CLASSIFICATION
  • PATTERNS
  • SALMON
  • DNA
  • PRODUCTS
  • fish
  • spoilage
  • vacuum-packaging
  • lactic acid bacteria
  • restriction endonuclease analysis
  • rainbow trout cold-smoked, fish, spoilage, vacuum-packaged
  • storage temperature
  • Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Gram-Positive Cocci [07700] Leuconostoc citreum food contaminant Leuconostoc mesenteroides mesenteroides food contaminant
  • Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Regular Nonsporing Gram-Positive Rods [07830] Lactobacillus plantarum food contaminant
  • Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteria [05000] lactic acid bacteria food contaminant
  • rRNA ribosomal RNA
  • 10050, Biochemistry methods - General
  • 10060, Biochemistry studies - General
  • 13502, Food technology - General and methods
  • 23001, Temperature - General measurement and methods
  • 31500, Genetics of bacteria and viruses
  • 39008, Food microbiology - General and miscellaneous
  • ribotyping analytical method
  • Foods
  • BACTERIA
  • LACTIC ACID BACTERIA
  • LACTOBACILLUS
  • LEUCONOSTOC
  • ONCORHYNCHUS MYKISS
  • RAINBOW TROUT
  • SMOKED FOODS
  • TROUT
  • Fish and fish products
  • Fish and marine products

Fingerprint Dive into the research topics of 'Characterisation of lactic acid bacteria from spoiled, vacuum-packaged, cold-smoked rainbow trout using ribotyping'. Together they form a unique fingerprint.

Cite this