CHARACTERISATION OF CELL-MEDIATED IMMUNE RESPONSE IN PIGS IN A CLINICAL CHALLENGE EXPERIMENT OF A VACCINE AGAINST MYCOPLASMA HYOSYNOVIAE

Josephine Skovgaard Rasmussen, Ulla Riber, Klara Tølbøll Lauritsen, Jeanne Toft Jakobsen, Sardar Ahmad, Gregers Jungersen

    Research output: Contribution to conferenceConference abstract for conferenceResearch

    Abstract

    Mycoplasma hyosynoviae (Mhs) is an extracellular parasitizing bacterium that causes arthritis in swine. The infection is widespread in modern pig production. An effective immunoprophylaxis is not available. In an experimental infection study (Lauritsen, K. T., et al., 2009. 3rd EVIW Poster, Berlin (Germany) September 10-13, 2009), testing a new vaccine (formalin inactivated Mhs and EMULSIGEN-BCL® (MVP Laboratories)), cell-mediated immune response was measured in the vaccine group (n=13) and not in the placebo group (n=13). In an IFN-γ assay, where whole-blood was co-cultured with Mhs-antigen and recombinant IL-18, significantly higher level of IFN-γ was produced in the vaccine group compared to the placebo group one day before challenge with Mhs. In contrast, significantly higher level of IFN-γ was measured in the placebo group compared to the vaccine group at day 6 after challenge. The latter could be due to increased systemic infection in the placebo group. Cell-mediated immune response was further characterised by four colour flow cytometry analysis of peripheral blood mononuclear cells (PBMCs) before Mhs challenge (day -1) and at days 6 and 9 after challenge. IFN-γ producing cells were found to be CD4 and especially CD4CD8 double positive T-cells simultaneously expressing CD25. Interestingly, the proportion of CD4CD8 double positive T-cells within the total population of CD4 positive cells increased in the vaccine group after challenge, indicating that generation of specific T-cell memory had occurred.
    Original languageEnglish
    Publication date2009
    Publication statusPublished - 2009
    Event3rd European Veterinary Immunology Workshop - Berlin, Germany
    Duration: 1 Sep 200913 Sep 2009
    Conference number: 3rd

    Conference

    Conference3rd European Veterinary Immunology Workshop
    Number3rd
    Country/TerritoryGermany
    CityBerlin
    Period01/09/200913/09/2009

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