Changes of in vivo fluxes through central metabolic pathways during the production of nystatin by Streptomyces noursei in batch culture

E. Jonsbu, Bjarke Christensen, Jens Nielsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The central carbon metabolism of the nystatin-producing strain Streptomyces noursei ATCC 11455 was evaluated by C-13-labelling experiments. A batch fermentation was examined during the idiophase by GC-MS measurements of the labelling patterns of amino acids in the biomass. The labelling patterns of the amino acids and calculated fluxes of the central metabolism showed that changes in the primary and secondary metabolisms occurred simultaneously. Changes in the profiles for the integrated fluxes showed a decreased flux through the pentose phosphate pathway and an increased flux in the tricarboxylic acid cycle relative to the glucose uptake rate when the culture entered a phase with reduced specific growth rate and enhanced nystatin yield. The flux through the pentose phosphate pathway seemed to be adjusted according to the NADPH requirement during the different phases of the batch fermentation.
    Original languageEnglish
    JournalApplied Microbiology and Biotechnology
    Volume56
    Issue number1-2
    Pages (from-to)93-100
    ISSN0175-7598
    DOIs
    Publication statusPublished - 2001

    Keywords

    • cpb

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