TY - JOUR
T1 - Challenge studies of European stocks of redfin perch, Perca fluviatilis L., and rainbow trout, Oncorhynchus mykiss (Walbaum), with epizootic haematopoietic necrosis virus
AU - Ariel, Ellen
AU - Jensen, Ann Britt Bang
PY - 2009
Y1 - 2009
N2 - A challenge model for comparison of the virulence
of epizootic haematopoietic necrosis virus (EHNV)
to European stock of redfin perch, Perca fluviatilis
L., and rainbow trout, Oncorhynchus mykiss
(Walbaum), was tested. The model investigated
intraperitoneal (IP), bath and cohabitation routes at
10, 15 and 20 C for 5–6 g fish and 15 C for 20 g
perch. In the IP challenges of perch, significant
mortality occurred at 15 C and 20 C. In challenge
trials for rainbow trout, significant mortalities were
observed in IP and bath challenges at 20 C. The
mortality observed in IP-challenged 20 g perch was
not significantly different from that recorded for 6 g
fish challenged IP. No significant mortality was
observed in any other treatment groups. Re-isolation
of ranavirus was confirmed by IFAT and was con-
sistently associated with dead or moribund fish in
the trial groups challenged with EHNV. The find-
ings indicate that EHNV does not pose a high risk
for wild perch and trout populations in Europe by
natural exposure. Mortality appears to be primarily a
function of environmental factors, with temperature
playing an important role, and not just the presence
of the virus in the fish.
AB - A challenge model for comparison of the virulence
of epizootic haematopoietic necrosis virus (EHNV)
to European stock of redfin perch, Perca fluviatilis
L., and rainbow trout, Oncorhynchus mykiss
(Walbaum), was tested. The model investigated
intraperitoneal (IP), bath and cohabitation routes at
10, 15 and 20 C for 5–6 g fish and 15 C for 20 g
perch. In the IP challenges of perch, significant
mortality occurred at 15 C and 20 C. In challenge
trials for rainbow trout, significant mortalities were
observed in IP and bath challenges at 20 C. The
mortality observed in IP-challenged 20 g perch was
not significantly different from that recorded for 6 g
fish challenged IP. No significant mortality was
observed in any other treatment groups. Re-isolation
of ranavirus was confirmed by IFAT and was con-
sistently associated with dead or moribund fish in
the trial groups challenged with EHNV. The find-
ings indicate that EHNV does not pose a high risk
for wild perch and trout populations in Europe by
natural exposure. Mortality appears to be primarily a
function of environmental factors, with temperature
playing an important role, and not just the presence
of the virus in the fish.
U2 - 10.1111/j.1365-2761.2009.01088.x
DO - 10.1111/j.1365-2761.2009.01088.x
M3 - Journal article
C2 - 19702625
SN - 0140-7775
VL - 32
SP - 1017
EP - 1025
JO - Journal of Fish Diseases
JF - Journal of Fish Diseases
IS - 12
ER -