Cellulose immobilization strategies for paper-based immunoassays

Selma B. Belfakir*, Winnie E. Svendsen, Andreas H. Laustsen, Georgina M.S. Ross*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

106 Downloads (Pure)

Abstract

Paper-based analytical devices (PADs) enable the affordable, easy, rapid, and reliable detection of a range of analytes at the point-of-care. Cellulose is a versatile substrate for antibody attachment. However, how immunoreagents are immobilized onto cellulose plays an important role in assay performance. To provide an overview of the different antibody immobilization strategies used for developing PADs, we here critically review the existing literature from the last decade (2013-2023). First, we introduce cellulose as a substrate and summarize the different fabrication techniques for developing PADs. Thereafter, we delve into how antibodies are immobilized onto cellulose passively, covalently, or by affinity linkage. Affinity-based immobilization is further explored in a case study focused on using recombinant bifunctional proteins with cellulose-binding modules to anchor immunoreagents to cellulose. The final section discusses the validation and sustainable development of PADs addressing the existing and future challenges associated with developing such devices.
Original languageEnglish
Article number117770
JournalTrends in Analytical Chemistry
Volume176
Number of pages13
ISSN0165-9936
DOIs
Publication statusPublished - 2024

Keywords

  • Immobilization
  • Paper-based diagnostics
  • Antibody
  • Covalent
  • Cellulose-binding modules
  • Orientation
  • Sustainability

Fingerprint

Dive into the research topics of 'Cellulose immobilization strategies for paper-based immunoassays'. Together they form a unique fingerprint.

Cite this