Cellulase and Macerozyme-PEG-mediated Transformation of Moss Protoplasts

Rituraj Batth, Ilenuta Simina Cuciurean, Satish Kumar Kodiripaka, Sarah Skovlund Rothman, Caroline Greisen, Henrik Toft Simonsen*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

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Abstract

This protocol describes the generation of protoplasts from protonemal tissue of the moss Physcomitrium patens (syn. Physcomitrella patens), using Cellulase ONOZUKA R10 and Macerozyme R10, followed by polyethylene glycol (PEG) mediated transformation. The protonemal tissue grown in liquid suspension was harvested and treated with enzyme cocktails mix of 1.5% Cellulase ONOZUKA R10 and 0.5% Macerozyme R10 to generate 1,8 million protoplasts within 3 h.
Original languageEnglish
Article numbere3782
JournalBio-protocol
Volume11
Issue number2
Number of pages12
ISSN2331-8325
DOIs
Publication statusPublished - 2021

Keywords

  • Moss
  • Physcomitrium patens (syn. Physcomitrella patens)
  • Protoplastation
  • Cellulase
  • Macerozyme

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