Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus

G. Scapigliati, F. Buonocore, E. Randelli, D. Casani, S. Meloni, G. Zarletti, M. Tiberi, D. Pietretti, I. Boschi, M. Manchado, B. Martin-Antonio, R. Jimenez-Cantizano, G. Bovo, F. Borghesan, Niels Lorenzen, Katja Einer-Jensen, S. Adams, K. Thompson, C. Alonso, J. Bejar & 2 others J.J. Borrego, M.C. Alvarez

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Naïve sea bass juveniles (38.4 ± 4.5 g) were intramuscularly infected with a sublethal dose of betanodavirus isolate 378/I03, followed after 43 days by a similar boosting. This infection resulted in an overall mortality of 7.6%. At various intervals, sampling of fish tissues was performed to investigate: i) B and T lymphocyte content in organs and tissues; ii), proliferation of leucocytes re-stimulated in vitro with inactivated virus; iii) presence of serum antibody specific for betanodavirus; iv) expression of genes coding for the following immunoregulatory molecules involved in innate and acquired responses: type I IFN, Mx, IL-1, Cox-2; IL-10, TGF-β, TCRβ, CD4, CD8α, IgM, by using a quantitative PCR array system developed for sea bass. The obtained results showed a detectable increase of T cells and B cells in PBL during betanodavirus infection. Furthermore, leucocytes obtained from blood, head kidney, and gills showed a detectable “in vitro” increase in viability upon addition of inactivated viral particles, as determined by measuring intracellular ATP concentration. ELISA analysis of sera showed that exposure to nodavirus induced a low, but specific antibody titer measured 43 days after infection, despite the presence of measurable levels of natural antibody. Finally, a strong upregulation of genes coding for type I IFN, Mx, and IgM was identified after both infection and boosting. Interestingly, an upregulation of Cox-2 until boosting, and of TGF-β and IL-10 after boosting was also observed, while the other tested genes did not show any significant variations with respect to mock-treated fish. Overall, our work represents a first comprehensive analysis of cellular and molecular immune parameters in a fish species exposed to a pathogenic virus.
    Original languageEnglish
    JournalFish and Shellfish Immunology
    Issue number28
    Pages (from-to)303-311
    ISSN1050-4648
    DOIs
    Publication statusPublished - 2010

    Cite this

    Scapigliati, G., Buonocore, F., Randelli, E., Casani, D., Meloni, S., Zarletti, G., ... Alvarez, M. C. (2010). Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus. Fish and Shellfish Immunology, (28), 303-311. https://doi.org/10.1016/j.fsi.2009.11.008
    Scapigliati, G. ; Buonocore, F. ; Randelli, E. ; Casani, D. ; Meloni, S. ; Zarletti, G. ; Tiberi, M. ; Pietretti, D. ; Boschi, I. ; Manchado, M. ; Martin-Antonio, B. ; Jimenez-Cantizano, R. ; Bovo, G. ; Borghesan, F. ; Lorenzen, Niels ; Einer-Jensen, Katja ; Adams, S. ; Thompson, K. ; Alonso, C. ; Bejar, J. ; Borrego, J.J. ; Alvarez, M.C. / Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus. In: Fish and Shellfish Immunology. 2010 ; No. 28. pp. 303-311.
    @article{6f3c711146c644608634d03bc08f47c8,
    title = "Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus",
    abstract = "Na{\"i}ve sea bass juveniles (38.4 ± 4.5 g) were intramuscularly infected with a sublethal dose of betanodavirus isolate 378/I03, followed after 43 days by a similar boosting. This infection resulted in an overall mortality of 7.6{\%}. At various intervals, sampling of fish tissues was performed to investigate: i) B and T lymphocyte content in organs and tissues; ii), proliferation of leucocytes re-stimulated in vitro with inactivated virus; iii) presence of serum antibody specific for betanodavirus; iv) expression of genes coding for the following immunoregulatory molecules involved in innate and acquired responses: type I IFN, Mx, IL-1, Cox-2; IL-10, TGF-β, TCRβ, CD4, CD8α, IgM, by using a quantitative PCR array system developed for sea bass. The obtained results showed a detectable increase of T cells and B cells in PBL during betanodavirus infection. Furthermore, leucocytes obtained from blood, head kidney, and gills showed a detectable “in vitro” increase in viability upon addition of inactivated viral particles, as determined by measuring intracellular ATP concentration. ELISA analysis of sera showed that exposure to nodavirus induced a low, but specific antibody titer measured 43 days after infection, despite the presence of measurable levels of natural antibody. Finally, a strong upregulation of genes coding for type I IFN, Mx, and IgM was identified after both infection and boosting. Interestingly, an upregulation of Cox-2 until boosting, and of TGF-β and IL-10 after boosting was also observed, while the other tested genes did not show any significant variations with respect to mock-treated fish. Overall, our work represents a first comprehensive analysis of cellular and molecular immune parameters in a fish species exposed to a pathogenic virus.",
    author = "G. Scapigliati and F. Buonocore and E. Randelli and D. Casani and S. Meloni and G. Zarletti and M. Tiberi and D. Pietretti and I. Boschi and M. Manchado and B. Martin-Antonio and R. Jimenez-Cantizano and G. Bovo and F. Borghesan and Niels Lorenzen and Katja Einer-Jensen and S. Adams and K. Thompson and C. Alonso and J. Bejar and J.J. Borrego and M.C. Alvarez",
    year = "2010",
    doi = "10.1016/j.fsi.2009.11.008",
    language = "English",
    pages = "303--311",
    journal = "Fish and Shellfish Immunology",
    issn = "1050-4648",
    publisher = "Academic Press",
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    Scapigliati, G, Buonocore, F, Randelli, E, Casani, D, Meloni, S, Zarletti, G, Tiberi, M, Pietretti, D, Boschi, I, Manchado, M, Martin-Antonio, B, Jimenez-Cantizano, R, Bovo, G, Borghesan, F, Lorenzen, N, Einer-Jensen, K, Adams, S, Thompson, K, Alonso, C, Bejar, J, Borrego, JJ & Alvarez, MC 2010, 'Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus', Fish and Shellfish Immunology, no. 28, pp. 303-311. https://doi.org/10.1016/j.fsi.2009.11.008

    Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus. / Scapigliati, G.; Buonocore, F.; Randelli, E.; Casani, D.; Meloni, S.; Zarletti, G.; Tiberi, M.; Pietretti, D.; Boschi, I.; Manchado, M.; Martin-Antonio, B.; Jimenez-Cantizano, R.; Bovo, G.; Borghesan, F.; Lorenzen, Niels; Einer-Jensen, Katja; Adams, S.; Thompson, K.; Alonso, C.; Bejar, J.; Borrego, J.J.; Alvarez, M.C.

    In: Fish and Shellfish Immunology, No. 28, 2010, p. 303-311.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus

    AU - Scapigliati, G.

    AU - Buonocore, F.

    AU - Randelli, E.

    AU - Casani, D.

    AU - Meloni, S.

    AU - Zarletti, G.

    AU - Tiberi, M.

    AU - Pietretti, D.

    AU - Boschi, I.

    AU - Manchado, M.

    AU - Martin-Antonio, B.

    AU - Jimenez-Cantizano, R.

    AU - Bovo, G.

    AU - Borghesan, F.

    AU - Lorenzen, Niels

    AU - Einer-Jensen, Katja

    AU - Adams, S.

    AU - Thompson, K.

    AU - Alonso, C.

    AU - Bejar, J.

    AU - Borrego, J.J.

    AU - Alvarez, M.C.

    PY - 2010

    Y1 - 2010

    N2 - Naïve sea bass juveniles (38.4 ± 4.5 g) were intramuscularly infected with a sublethal dose of betanodavirus isolate 378/I03, followed after 43 days by a similar boosting. This infection resulted in an overall mortality of 7.6%. At various intervals, sampling of fish tissues was performed to investigate: i) B and T lymphocyte content in organs and tissues; ii), proliferation of leucocytes re-stimulated in vitro with inactivated virus; iii) presence of serum antibody specific for betanodavirus; iv) expression of genes coding for the following immunoregulatory molecules involved in innate and acquired responses: type I IFN, Mx, IL-1, Cox-2; IL-10, TGF-β, TCRβ, CD4, CD8α, IgM, by using a quantitative PCR array system developed for sea bass. The obtained results showed a detectable increase of T cells and B cells in PBL during betanodavirus infection. Furthermore, leucocytes obtained from blood, head kidney, and gills showed a detectable “in vitro” increase in viability upon addition of inactivated viral particles, as determined by measuring intracellular ATP concentration. ELISA analysis of sera showed that exposure to nodavirus induced a low, but specific antibody titer measured 43 days after infection, despite the presence of measurable levels of natural antibody. Finally, a strong upregulation of genes coding for type I IFN, Mx, and IgM was identified after both infection and boosting. Interestingly, an upregulation of Cox-2 until boosting, and of TGF-β and IL-10 after boosting was also observed, while the other tested genes did not show any significant variations with respect to mock-treated fish. Overall, our work represents a first comprehensive analysis of cellular and molecular immune parameters in a fish species exposed to a pathogenic virus.

    AB - Naïve sea bass juveniles (38.4 ± 4.5 g) were intramuscularly infected with a sublethal dose of betanodavirus isolate 378/I03, followed after 43 days by a similar boosting. This infection resulted in an overall mortality of 7.6%. At various intervals, sampling of fish tissues was performed to investigate: i) B and T lymphocyte content in organs and tissues; ii), proliferation of leucocytes re-stimulated in vitro with inactivated virus; iii) presence of serum antibody specific for betanodavirus; iv) expression of genes coding for the following immunoregulatory molecules involved in innate and acquired responses: type I IFN, Mx, IL-1, Cox-2; IL-10, TGF-β, TCRβ, CD4, CD8α, IgM, by using a quantitative PCR array system developed for sea bass. The obtained results showed a detectable increase of T cells and B cells in PBL during betanodavirus infection. Furthermore, leucocytes obtained from blood, head kidney, and gills showed a detectable “in vitro” increase in viability upon addition of inactivated viral particles, as determined by measuring intracellular ATP concentration. ELISA analysis of sera showed that exposure to nodavirus induced a low, but specific antibody titer measured 43 days after infection, despite the presence of measurable levels of natural antibody. Finally, a strong upregulation of genes coding for type I IFN, Mx, and IgM was identified after both infection and boosting. Interestingly, an upregulation of Cox-2 until boosting, and of TGF-β and IL-10 after boosting was also observed, while the other tested genes did not show any significant variations with respect to mock-treated fish. Overall, our work represents a first comprehensive analysis of cellular and molecular immune parameters in a fish species exposed to a pathogenic virus.

    U2 - 10.1016/j.fsi.2009.11.008

    DO - 10.1016/j.fsi.2009.11.008

    M3 - Journal article

    SP - 303

    EP - 311

    JO - Fish and Shellfish Immunology

    JF - Fish and Shellfish Immunology

    SN - 1050-4648

    IS - 28

    ER -