Cell motility, morphology, viability and proliferation in response to nanotopography on silicon black

Joanna M. Lopacinska, Cristian Gradinaru, Rafal Wierzbicki, Carsten Købler, Michael S. Schmidt, Martin T. Madsen, Maciej Skolimowski, Martin Dufva, Henrik Flyvbjerg, Kristian Mølhave

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Knowledge of cells' interactions with nanostructured materials is fundamental for bio-nanotechnology. We present results for how individual mouse fibroblasts from cell line NIH3T3 respond to highly spiked surfaces of silicon black that were fabricated by maskless reactive ion etching (RIE). We did standard measurements of cell viability, proliferation, and morphology on various surfaces. We also analyzed the motility of cells on the same surfaces, as recorded in time lapse movies of sparsely populated cell cultures. We find that motility and morphology vary strongly with nano-patterns, while viability and proliferation show little dependence on substrate type. We conclude that motility analysis can show a wide range of cell responses e. g. over a factor of two in cell speed to different nano-topographies, where standard assays, such as viability or proliferation, in the tested cases show much less variation of the order 10-20%.
    Original languageEnglish
    JournalNanoscale
    Volume4
    Issue number12
    Pages (from-to)3739-3745
    ISSN2040-3364
    DOIs
    Publication statusPublished - 2012

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