Cell Factory Engineering

Anne Mathilde Davy, Helene Faustrup Kildegaard, Mikael Rørdam Andersen

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Rational approaches to modifying cells to make molecules of interest are of substantial economic and scientific interest. Most of these efforts aim at the production of native metabolites, expression of heterologous biosynthetic pathways, or protein expression. Reviews of these topics have largely focused on individual strategies or cell types, but collectively they fall under the broad umbrella of a growing field known as cell factory engineering. Here we condense >130 reviews and key studies in the art into a meta-review of cell factory engineering. We identified 33 generic strategies in the field, all applicable to multiple types of cells and products, and proven successful in multiple major cell types. These apply to three major categories: production of native metabolites and/or bioactives, heterologous expression of biosynthetic pathways, and protein expression. This meta-review provides general strategy guides for the broad range of applications of rational engineering of cell factories.
Original languageEnglish
JournalCell Systems
Volume4
Issue number3
Pages (from-to)262-275
ISSN2405-4712
DOIs
Publication statusPublished - 2017

Keywords

  • Aspergillus
  • Bacillus subtilis
  • CHO cells
  • Escherichia coli
  • Pichia
  • Saccharomyces cerevisiae
  • cell factory
  • cell factory engineering
  • metabolic engineering
  • protein secretion

Cite this

Davy, Anne Mathilde ; Kildegaard, Helene Faustrup ; Andersen, Mikael Rørdam. / Cell Factory Engineering. In: Cell Systems. 2017 ; Vol. 4, No. 3. pp. 262-275.
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Davy, AM, Kildegaard, HF & Andersen, MR 2017, 'Cell Factory Engineering', Cell Systems, vol. 4, no. 3, pp. 262-275. https://doi.org/10.1016/j.cels.2017.02.010

Cell Factory Engineering. / Davy, Anne Mathilde; Kildegaard, Helene Faustrup; Andersen, Mikael Rørdam.

In: Cell Systems, Vol. 4, No. 3, 2017, p. 262-275.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Cell Factory Engineering

AU - Davy, Anne Mathilde

AU - Kildegaard, Helene Faustrup

AU - Andersen, Mikael Rørdam

PY - 2017

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AB - Rational approaches to modifying cells to make molecules of interest are of substantial economic and scientific interest. Most of these efforts aim at the production of native metabolites, expression of heterologous biosynthetic pathways, or protein expression. Reviews of these topics have largely focused on individual strategies or cell types, but collectively they fall under the broad umbrella of a growing field known as cell factory engineering. Here we condense >130 reviews and key studies in the art into a meta-review of cell factory engineering. We identified 33 generic strategies in the field, all applicable to multiple types of cells and products, and proven successful in multiple major cell types. These apply to three major categories: production of native metabolites and/or bioactives, heterologous expression of biosynthetic pathways, and protein expression. This meta-review provides general strategy guides for the broad range of applications of rational engineering of cell factories.

KW - Aspergillus

KW - Bacillus subtilis

KW - CHO cells

KW - Escherichia coli

KW - Pichia

KW - Saccharomyces cerevisiae

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KW - cell factory engineering

KW - metabolic engineering

KW - protein secretion

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DO - 10.1016/j.cels.2017.02.010

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JO - Cell Systems

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