CDNA cloning, characterization and expression of an endosperm-specific barley peroxidase

Søren Kjærsgård Rasmussen, K.G. Welinder, J. Hejgaard

    Research output: Contribution to journalJournal articleResearch


    A barley peroxidase (BP 1) of pI ca. 8.5 and M(r) 37000 has been purified from mature barley grains. Using antibodies towards peroxidase BP 1, a cDNA clone (pcR7) was isolated from cDNA expression library. The nucleotide sequence of pcR7 gave a derived amino acid sequence identical to the 158 C-terminal amino acid residues of mature BP 1. The clone pcR7 encodes an additional C-terminal sequence of 22 residues, which apparently are removed during processing. BP 1 is less than 50% identical to other sequenced plant peroxidases. Analyses of RNA and protein from aleurone, endosperm and embryo tissue showed maximal expression 15 days after flowering, and high levels were found only in the endosperm. BP 1 was not expressed in the leaves.
    Original languageEnglish
    JournalPlant Molecular Biology
    Issue number2
    Pages (from-to)317-327
    Publication statusPublished - 1991


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