CD14 hi HLA-DR dim macrophages, with a resemblance to classical blood monocytes, dominate inflamed mucosa in Crohn’s disease

Susanne Thiesen, Sabina Janciauskiene, Heli Uronen-Hansson, William Winston Agace, Carl-Magnus Hogerkorp, Pieter Spee, Katarina Hakansson, Katarina Hakansson

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Intestinal MΦ play an important role in maintaining guthomeostasis. However, little is known about thesecells, their precursors, and their role in intestinal inflammation.Here, we characterize the CD14 mononuclear cell populations in intestinal mucosa and blood in patients with CD. Among the LP CD14+ MΦ, we identifiedthree distinct HLA-DR+ -expressing subsets. Compared with uninflamed, inflamed mucosa contained a markedincrease in the proportion of the CD14hiHLA-DRdim cellular subset. This subset resembled the classical bloodmonocytes with low CD16, HLA-DR, and CX3CR1 expression. Classical monocytes migrated efficiently towardCCL2 and released the highest levels of MMP-1and proinflammatory cytokines when stimulated withimmune complexes or LPS. Our findings strongly suggestthat it is the classical and not the intermediate ornonclassical monocytes that are the precursors tothe dominating intestinal CD14hiHLA-DRdim subset.This enhances our understanding of CD pathologyand may provide new options in treatment.
Original languageEnglish
JournalJournal of Leukocyte Biology
Volume95
Issue number3
Pages (from-to)531-541
ISSN0741-5400
DOIs
Publication statusPublished - 2014
Externally publishedYes

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