Abstract
A highly efficient method for functionalizing nanoparticles and directly quantifying conjugation efficiency and ligand surface density has been developed. Attachment of 3-azido-modifed RGD-peptides to PEGylated liposomes was achieved by using Cu-free click conditions. Upon coupling a fluorophore is formed, which could be utilized to monitor conjugation efficiency and the obtained ligand surface density in situ, without prior purification.
Original language | English |
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Journal | Chemistry - A European Journal |
Volume | 17 |
Issue number | 12 |
Pages (from-to) | 3326-3331 |
ISSN | 0947-6539 |
DOIs | |
Publication status | Published - 2011 |
Keywords
- Coumarin
- Surface functionalization
- Nanoparticles
- Lposomes
- Click chemistry