Catalyst-Free Conjugation and In Situ Quantification of Nanoparticle Ligand Surface Density Using Fluorogenic Cu-Free Click Chemistry

Rasmus Irming Jølck, Honghao Sun, Rolf Henrik Berg, Thomas Lars Andresen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A highly efficient method for functionalizing nanoparticles and directly quantifying conjugation efficiency and ligand surface density has been developed. Attachment of 3-azido-modifed RGD-peptides to PEGylated liposomes was achieved by using Cu-free click conditions. Upon coupling a fluorophore is formed, which could be utilized to monitor conjugation efficiency and the obtained ligand surface density in situ, without prior purification.
    Original languageEnglish
    JournalChemistry - A European journal
    Volume17
    Issue number12
    Pages (from-to)3326-3331
    ISSN0947-6539
    DOIs
    Publication statusPublished - 2011

    Keywords

    • Coumarin
    • Surface functionalization
    • Nanoparticles
    • Lposomes
    • Click chemistry

    Fingerprint Dive into the research topics of 'Catalyst-Free Conjugation and In Situ Quantification of Nanoparticle Ligand Surface Density Using Fluorogenic Cu-Free Click Chemistry'. Together they form a unique fingerprint.

    Cite this