Carbon Nanotube-Based Separation Columns for Microchip Electrochromatography

Klaus Bo Mogensen, B. Delacourt, Jörg Peter Kutter

    Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

    Abstract

    Fabrication of the stationary phase for microchip chromatography is most often done by packing of the individual separation channel after fabrication of the microfluidic chip, which is a very time-consuming and costly process (Kutter. J Chromatogr A 1221:72–82, 2012). Here, we describe in detail the fabrication and operation protocols for devices with microfabricated carbon nanotube stationary phases for reverse-phase chromatography. In this protocol, the lithographically defined stationary phase is fabricated in the channel before bonding of a lid, thereby circumventing the difficult packaging procedures used in more conventional protocols.
    Original languageEnglish
    Title of host publicationMicrochip Capillary Electrophoresis Protocols
    PublisherSpringer
    Publication date2015
    Pages149-159
    Chapter13
    ISBN (Print)978-1-4939-2352-6
    Publication statusPublished - 2015
    SeriesMethods in Molecular Biology
    Volume1274
    ISSN1064-3745

    Keywords

    • Microchip electrochromatography
    • Carbon nanotubes
    • Nonaqueous separations
    • Microfabrication

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