Fabrication of the stationary phase for microchip chromatography is most often done by packing of the individual separation channel after fabrication of the microfluidic chip, which is a very time-consuming and costly process (Kutter. J Chromatogr A 1221:72–82, 2012). Here, we describe in detail the fabrication and operation protocols for devices with microfabricated carbon nanotube stationary phases for reverse-phase chromatography. In this protocol, the lithographically defined stationary phase is fabricated in the channel before bonding of a lid, thereby circumventing the difficult packaging procedures used in more conventional protocols.
|Title of host publication||Microchip Capillary Electrophoresis Protocols|
|Publication status||Published - 2015|
|Series||Methods in Molecular Biology|
- Microchip electrochromatography
- Carbon nanotubes
- Nonaqueous separations
Mogensen, K. B., Delacourt, B., & Kutter, J. P. (2015). Carbon Nanotube-Based Separation Columns for Microchip Electrochromatography. In Microchip Capillary Electrophoresis Protocols (pp. 149-159). Springer. Methods in Molecular Biology, Vol.. 1274