Carbohydrate structural determination by NMR spectroscopy: Modern methods and limitations

Jens Øllgaard Duus; Charlotte Held Gotfredsen; Klaus Bock

doi:10.1021/cr990302n

Carbohydrate structural determination by NMR spectroscopy: Modern methods and limitations

Carlsberg Research Center

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The general problem of structural assignment of complex carbohydrate structures by NMR spectroscopy was reviewed. It was claimed that carbohydrates contains the hidden code to biological recognition. It was shown that the actual sensitivity obtained in a nanoprobe operating at 500 MHz was similar to the sensitivity obtained in a 3-mm probe on an 800MHz instrument, off course without the same spectral dispersion. Therefore, for many oligosaccharide samples it is more advantageous to use the new probe technology and the many new pulse sequences than going to higher field strength with the associated substantially much higher expense both in investment and running cost.

Original language	English
Journal	Chemical Reviews
Volume	100
Issue number	12
Pages (from-to)	4589-4614
ISSN	0009-2665
DOIs	https://doi.org/10.1021/cr990302n
Publication status	Published - 2000
Externally published	Yes

Keywords

Carbon
Chemical bonds
Computer simulation
Derivatives
Mass spectrometry
Molecular structure
Nuclear magnetic resonance spectroscopy
Polysaccharides
Proteins
Protons
Spectroscopic analysis
Structural analysis
Carbohydrates
Magnet technology
Nucleosides
Oligosaccharides
Sugar nucleotides
CHEMISTRY
NUCLEAR-MAGNETIC-RESONANCE
SPIN-COUPLING-CONSTANTS
HIGH-RESOLUTION NMR
O-SPECIFIC POLYSACCHARIDE
RESIDUAL DIPOLAR COUPLINGS
PULSED-FIELD GRADIENTS
SOLID-PHASE-SYNTHESIS
ARTIFICIAL NEURAL-NETWORKS
E.COSY-TYPE MEASUREMENT
CHROMATOGRAPHY MASS-SPECTROMETRY
CARBOHYDRATES

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@article{89aa4f7f2f0148cf9124cb2b8ce4831d,

title = "Carbohydrate structural determination by NMR spectroscopy: Modern methods and limitations",

abstract = "The general problem of structural assignment of complex carbohydrate structures by NMR spectroscopy was reviewed. It was claimed that carbohydrates contains the hidden code to biological recognition. It was shown that the actual sensitivity obtained in a nanoprobe operating at 500 MHz was similar to the sensitivity obtained in a 3-mm probe on an 800MHz instrument, off course without the same spectral dispersion. Therefore, for many oligosaccharide samples it is more advantageous to use the new probe technology and the many new pulse sequences than going to higher field strength with the associated substantially much higher expense both in investment and running cost.",

keywords = "Carbon, Chemical bonds, Computer simulation, Derivatives, Mass spectrometry, Molecular structure, Nuclear magnetic resonance spectroscopy, Polysaccharides, Proteins, Protons, Spectroscopic analysis, Structural analysis, Carbohydrates, Magnet technology, Nucleosides, Oligosaccharides, Sugar nucleotides, CHEMISTRY, NUCLEAR-MAGNETIC-RESONANCE, SPIN-COUPLING-CONSTANTS, HIGH-RESOLUTION NMR, O-SPECIFIC POLYSACCHARIDE, RESIDUAL DIPOLAR COUPLINGS, PULSED-FIELD GRADIENTS, SOLID-PHASE-SYNTHESIS, ARTIFICIAL NEURAL-NETWORKS, E.COSY-TYPE MEASUREMENT, CHROMATOGRAPHY MASS-SPECTROMETRY, CARBOHYDRATES",

author = "Duus, \{Jens {\O}llgaard\} and Gotfredsen, \{Charlotte Held\} and Klaus Bock",

year = "2000",

doi = "10.1021/cr990302n",

language = "English",

volume = "100",

pages = "4589--4614",

journal = "Chemical Reviews",

issn = "0009-2665",

publisher = "American Chemical Society",

number = "12",

}

TY - JOUR

T1 - Carbohydrate structural determination by NMR spectroscopy: Modern methods and limitations

AU - Duus, Jens Øllgaard

AU - Gotfredsen, Charlotte Held

AU - Bock, Klaus

PY - 2000

Y1 - 2000

N2 - The general problem of structural assignment of complex carbohydrate structures by NMR spectroscopy was reviewed. It was claimed that carbohydrates contains the hidden code to biological recognition. It was shown that the actual sensitivity obtained in a nanoprobe operating at 500 MHz was similar to the sensitivity obtained in a 3-mm probe on an 800MHz instrument, off course without the same spectral dispersion. Therefore, for many oligosaccharide samples it is more advantageous to use the new probe technology and the many new pulse sequences than going to higher field strength with the associated substantially much higher expense both in investment and running cost.

AB - The general problem of structural assignment of complex carbohydrate structures by NMR spectroscopy was reviewed. It was claimed that carbohydrates contains the hidden code to biological recognition. It was shown that the actual sensitivity obtained in a nanoprobe operating at 500 MHz was similar to the sensitivity obtained in a 3-mm probe on an 800MHz instrument, off course without the same spectral dispersion. Therefore, for many oligosaccharide samples it is more advantageous to use the new probe technology and the many new pulse sequences than going to higher field strength with the associated substantially much higher expense both in investment and running cost.

KW - Carbon

KW - Chemical bonds

KW - Computer simulation

KW - Derivatives

KW - Mass spectrometry

KW - Molecular structure

KW - Nuclear magnetic resonance spectroscopy

KW - Polysaccharides

KW - Proteins

KW - Protons

KW - Spectroscopic analysis

KW - Structural analysis

KW - Carbohydrates

KW - Magnet technology

KW - Nucleosides

KW - Oligosaccharides

KW - Sugar nucleotides

KW - CHEMISTRY

KW - NUCLEAR-MAGNETIC-RESONANCE

KW - SPIN-COUPLING-CONSTANTS

KW - HIGH-RESOLUTION NMR

KW - O-SPECIFIC POLYSACCHARIDE

KW - RESIDUAL DIPOLAR COUPLINGS

KW - PULSED-FIELD GRADIENTS

KW - SOLID-PHASE-SYNTHESIS

KW - ARTIFICIAL NEURAL-NETWORKS

KW - E.COSY-TYPE MEASUREMENT

KW - CHROMATOGRAPHY MASS-SPECTROMETRY

KW - CARBOHYDRATES

U2 - 10.1021/cr990302n

DO - 10.1021/cr990302n

M3 - Journal article

SN - 0009-2665

VL - 100

SP - 4589

EP - 4614

JO - Chemical Reviews

JF - Chemical Reviews

IS - 12

ER -

Carbohydrate structural determination by NMR spectroscopy: Modern methods and limitations

Abstract

Keywords

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