Carbohydrate structural determination by NMR spectroscopy: Modern methods and limitations

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Abstract

The general problem of structural assignment of complex carbohydrate structures by NMR spectroscopy was reviewed. It was claimed that carbohydrates contains the hidden code to biological recognition. It was shown that the actual sensitivity obtained in a nanoprobe operating at 500 MHz was similar to the sensitivity obtained in a 3-mm probe on an 800MHz instrument, off course without the same spectral dispersion. Therefore, for many oligosaccharide samples it is more advantageous to use the new probe technology and the many new pulse sequences than going to higher field strength with the associated substantially much higher expense both in investment and running cost.
Original languageEnglish
JournalChemical Reviews
Volume100
Issue number12
Pages (from-to)4589-4614
ISSN0009-2665
DOIs
Publication statusPublished - 2000
Externally publishedYes

Keywords

  • Carbon
  • Chemical bonds
  • Computer simulation
  • Derivatives
  • Mass spectrometry
  • Molecular structure
  • Nuclear magnetic resonance spectroscopy
  • Polysaccharides
  • Proteins
  • Protons
  • Spectroscopic analysis
  • Structural analysis
  • Carbohydrates
  • Magnet technology
  • Nucleosides
  • Oligosaccharides
  • Sugar nucleotides
  • CHEMISTRY
  • NUCLEAR-MAGNETIC-RESONANCE
  • SPIN-COUPLING-CONSTANTS
  • HIGH-RESOLUTION NMR
  • O-SPECIFIC POLYSACCHARIDE
  • RESIDUAL DIPOLAR COUPLINGS
  • PULSED-FIELD GRADIENTS
  • SOLID-PHASE-SYNTHESIS
  • ARTIFICIAL NEURAL-NETWORKS
  • E.COSY-TYPE MEASUREMENT
  • CHROMATOGRAPHY MASS-SPECTROMETRY
  • CARBOHYDRATES

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